PXD016474 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic comparison study between cancer cachexia muscle of C26 and CT mice |
Description | Background Loss of skeletal muscle mass in advanced cancer is recognized as an independent predictor of mortality. Mechanisms involved in this wasting process and parameters for early diagnosis are still lacking. As skeletal muscle is considered as a secretory organ, the aim of this present experimental work was to characterize the changes in muscle proteome and secretome associated with cancer-induced cachexia to better understand cellular mechanisms involved in this wasting process and to identify secreted proteins which might reflect the ongoing muscle atrophy process. Methods We investigated first the changes in the muscle proteome associated with cancer-induced cachexia by using differential label-free proteomic analysis on muscle of the C26 mouse model. The differentially abundant proteins were submitted to sequential bioinformatic secretomic analysis in order to identify potentially secreted proteins. Selected reaction monitoring and Western blotting were used to verify the presence of candidate proteins at the circulating level. Their muscle source was demonstrated by assessing their gene expression in skeletal muscle and in cultured myotubes. Finally, we also investigated their regulation in muscle cells. Alterations in several molecular pathways potentially involved in muscle atrophy were highlighted using Gene ontology enrichment analyses. Results Our results revealed a dramatic increased production (2-to 25-fold) by the muscle of several acute phase reactants (APR: Haptoglobin, Serpina3n, Complement C3, Serum amyloid A1) which are also released in the circulation during C26 cancer cachexia. Their production was confirmed in other preclinical models of cancer cachexia as well as in cancer patients. The muscular origin of these APR was demonstrated by their increased expression in skeletal muscle and myotubes. Glucocorticoids and pro-inflammatory cytokines contribute directly to their increased expression in muscle cells in vitro, while the role of IL-6 in the muscular induction of these APR was demonstrated in vivo. Conclusions Cancer is associated with marked changes in muscle secretome during muscle wasting. Our study demonstrates a marked increased production of APR by skeletal muscle in pre-clinical models of cancer cachexia and in cancer patients. Further studies are required to unravel the potential role of these proteins in muscle atrophy and their interest as biomarkers of cancer cachexia. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:14:20.005.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Marie-Alice Meuwis |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-11-27 03:43:22 | ID requested | |
1 | 2020-10-28 06:46:06 | announced | |
2 | 2020-11-09 04:40:39 | announced | 2020-11-09: Updated publication reference for PubMed record(s): 33142864. |
⏵ 3 | 2024-10-22 05:14:20 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.3390/cancers12113221; |
Massart IS, Paulissen G, Loumaye A, Lause P, P, ö, tgens SA, Thibaut MM, Balan E, Deldicque L, Atfi A, Louis E, Gruson D, Bindels LB, Meuwis MA, Thissen JP, Marked Increased Production of Acute Phase Reactants by Skeletal Muscle during Cancer Cachexia. Cancers (Basel), 12(11):(2020) [pubmed] |
Keyword List
submitter keyword: cancer,Biomarkers, C26 mice, cachexia, muscle, atrophy |
Contact List
Meuwis Marie-Alice |
contact affiliation | Translational Gastroenterology,GIGA institute,University of Liège, Belgium Hepato-Gastroenterology and Digestive Oncology Dep., University hospital of Liège, BE |
contact email | marie-alice.meuwis@uliege.be |
lab head | |
Marie-Alice Meuwis |
contact affiliation | translational gastroenterology laboratory University of Liege, GIGA institute: university hospital CHU Liège, BE |
contact email | marie-alice.meuwis@uliege.be |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD016474 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016474
- Label: PRIDE project
- Name: Proteomic comparison study between cancer cachexia muscle of C26 and CT mice