PXD016473

PXD016473 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of serum small extracellular vesicles of individual mice for longitudinal investigations
Description	Characterization of small extracellular vesicles (sEV) from individual mice for the longitudinal investigation of proteome changes in a mouse model of Glioblastoma Multiforme (GBM). We first compared precipitation and size exclusion chromatography (SEC) for sEV purification from 100ul of mouse serum. We then optimized SEC-based procedure for the isolation of sEV’s from 50ul of mouse serum. The optimized procedure was then applied to compare the sEV proteomes for the longitudinal analysis of the GBM-mouse model.
HostingRepository	PRIDE
AnnounceDate	2020-11-30
AnnouncementXML	Submission_2020-11-30_00:22:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Federica Anastasi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-27 03:43:14	ID requested
⏵ 1	2020-11-30 00:22:26	announced

Anastasi F, Greco F, Dilillo M, Vannini E, Cappello V, Baroncelli L, Costa M, Gemmi M, Caleo M, McDonnell LA, Proteomics analysis of serum small extracellular vesicles for the longitudinal study of a glioblastoma multiforme mouse model. Sci Rep, 10(1):20498(2020) [pubmed]

submitter keyword: Small extracellular vesicles, proteomics, glioblastoma, serum, mouse, size exclusion chromatography, biomarker discovery

Liam A. McDonnell
contact affiliation	Fondazione Pisana per la Scienza ONLUS, 56107 San Giuliano Terme, Pisa, Italy Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands
contact email	liam@fpscience.it
lab head
Federica Anastasi
contact affiliation	Scuola Normale Superiore
contact email	federica.anastasi@sns.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD016473

PRIDE project URI

• PRIDE
  1. PXD016473
     1. Label: PRIDE project
     2. Name: Proteomic analysis of serum small extracellular vesicles of individual mice for longitudinal investigations