PXD016458

PXD016458 is an original dataset announced via ProteomeXchange.

Title	AP-MS analysis of human RIOK1 and USP16
Description	To discover novel factors involved in the final steps of 40S ribosomal subunit biogenesis in human cells, we isolated late cytoplasmic 40S subunit precursors by affinity purification (AP) of the assembly factor RIOK1. Subsequent analysis of their composition by mass spectrometry (MS) led to identification of the deubiquitinase USP16. AP-MS of USP16 confirms that it is a component of late cytoplasmic pre-40S particles and we further show that USP16 possesses a new, ribosome-associated function.
HostingRepository	PRIDE
AnnounceDate	2020-03-04
AnnouncementXML	Submission_2020-05-27_05:23:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ludovic Gillet
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-27 00:59:18	ID requested
1	2020-03-04 00:06:27	announced
⏵ 2	2020-05-27 05:23:06	announced	2020-05-27: Updated publication reference for PubMed record(s): 32129764.

Montellese C, van den Heuvel J, Ashiono C, D, ö, rner K, Melnik A, Jonas S, Zemp I, Picotti P, Gillet LC, Kutay U, USP16 counteracts mono-ubiquitination of RPS27a and promotes maturation of the 40S ribosomal subunit. Elife, 9():(2020) [pubmed]

curator keyword: Biological

submitter keyword: AP-MS, RIOK1, USP16, DDA

Ulrike Kutay
contact affiliation	Department of Biology, Institute of Biochemistry, ETH Zurich, Switzerland
contact email	ulrike.kutay@bc.biol.ethz.ch
lab head
Ludovic Gillet
contact affiliation	ETH Zurich
contact email	gillet@imsb.biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD016458

PRIDE project URI

• PRIDE
  1. PXD016458
     1. Label: PRIDE project
     2. Name: AP-MS analysis of human RIOK1 and USP16