PXD016458 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | AP-MS analysis of human RIOK1 and USP16 |
Description | To discover novel factors involved in the final steps of 40S ribosomal subunit biogenesis in human cells, we isolated late cytoplasmic 40S subunit precursors by affinity purification (AP) of the assembly factor RIOK1. Subsequent analysis of their composition by mass spectrometry (MS) led to identification of the deubiquitinase USP16. AP-MS of USP16 confirms that it is a component of late cytoplasmic pre-40S particles and we further show that USP16 possesses a new, ribosome-associated function. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:00:31.584.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ludovic Gillet |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-11-27 00:59:18 | ID requested | |
1 | 2020-03-04 00:06:27 | announced | |
2 | 2020-05-27 05:23:06 | announced | 2020-05-27: Updated publication reference for PubMed record(s): 32129764. |
⏵ 3 | 2024-10-22 05:00:32 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.7554/elife.54435; |
Montellese C, van den Heuvel J, Ashiono C, D, รถ, rner K, Melnik A, Jonas S, Zemp I, Picotti P, Gillet LC, Kutay U, USP16 counteracts mono-ubiquitination of RPS27a and promotes maturation of the 40S ribosomal subunit. Elife, 9():(2020) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: USP16,AP-MS, RIOK1, DDA |
Contact List
Ulrike Kutay |
contact affiliation | Department of Biology, Institute of Biochemistry, ETH Zurich, Switzerland |
contact email | ulrike.kutay@bc.biol.ethz.ch |
lab head | |
Ludovic Gillet |
contact affiliation | ETH Zurich |
contact email | gillet@imsb.biol.ethz.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD016458 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016458
- Label: PRIDE project
- Name: AP-MS analysis of human RIOK1 and USP16