PXD016430

PXD016430 is an original dataset announced via ProteomeXchange.

Title	MiR-29 coordinates age-dependent plasticity brakes in the adult visual cortex
Description	Visual cortical circuits show profound plasticity during early life and are later stabilized by molecular "brakes" limiting excessive circuit rewiring beyond a critical period. How the appearance of these factors is coordinated during the transition from development to adulthood remains unknown. We analyzed the role of miR-29a, a miRNA targeting factors involved in several important pathways for plasticity such as extracellular matrix and chromatin regulation. We found that visual cortical miR-29a expression in the visual cortex dramatically increases with age, but it is not experience-dependent. Precocious high levels of miR-29a induced by targeted intracortical injections of a miR-29a mimic blocked ocular dominance plasticity and caused an early appearance of perineuronal nets. Conversely, inhibition of miR-29a in adult mice using LNA antagomirs activated ocular dominance plasticity, reduced perineuronal net intensity and number, and changed their chemical composition restoring permissive low chondroitin 4-O-sulfation levels characteristic of juvenile mice. Activated adult plasticity had the typical functional and proteomic signature of juvenile plasticity. Transcriptomic and proteomic studies indicated that miR-29a manipulation regulates the expression of plasticity factors acting at different cellular levels, from chromatin regulation to synaptic organization and extracellular matrix remodeling. Intriguingly, the projection of miR-29a regulated gene dataset onto cell-specific transcriptomes revealed that parvalbumin-positive interneurons and oligodendrocytes were the most affected cells. Overall, miR29a is a master regulator of the age-dependent plasticity brakes promoting stability of visual cortical circuits.
HostingRepository	PRIDE
AnnounceDate	2020-11-04
AnnouncementXML	Submission_2020-11-04_02:35:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Erika Kelmer Sacramento
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	carbamoylated residue; monohydroxylated residue; acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-25 04:39:22	ID requested
⏵ 1	2020-11-04 02:35:02	announced

Napoli D, Lupori L, Mazziotti R, Sagona G, Bagnoli S, Samad M, Sacramento EK, Kirkpartick J, Putignano E, Chen S, Terzibasi Tozzini E, Tognini P, Baldi P, Kwok JC, Cellerino A, Pizzorusso T, MiR-29 coordinates age-dependent plasticity brakes in the adult visual cortex. EMBO Rep, 21(11):e50431(2020) [pubmed]

curator keyword: Biological

submitter keyword: miR-29, visual cortex, age-dependent plasticity

Alessandro Cellerino
contact affiliation	Leibniz Institute on Aging – Fritz Lipmann Institute (FLI), Jena, Germany. Scuola Normale Superiore, Pisa, Italy.
contact email	alessandro.cellerino@sns.it
lab head
Erika Kelmer Sacramento
contact affiliation	Leibniz Institute on Aging - Fritz Lipmann Institute
contact email	erika.kelmer@leibniz-fli.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD016430

PRIDE project URI

• PRIDE
  1. PXD016430
     1. Label: PRIDE project
     2. Name: MiR-29 coordinates age-dependent plasticity brakes in the adult visual cortex