PXD016410

PXD016410 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Ultrasensitive Top-Down Proteomics Analysis of Low Number of Mammalian Cells Using a Nanodroplet Sample Processing Platform
Description	We developed an ultrasensitive top down platform by incorporating a microfluidic sample preparation system, termed nanoPOTS (Nanodroplet Processing in One pot for Trace Samples), into a top down workflow. We determined that a unique combination of a nonionic detergent DDM with urea as a protein extraction buffer can significantly improve both protein extraction efficiency and sample recovery. The nanoPOTS-based top down platform can reproducibly and quantitatively identify ~170 to ~620 proteoforms from ~70 to ~770 HeLa cells containing < ~100 ng total proteins. Data was searched with TopPIC and MSPathFinder.
HostingRepository	MassIVE
AnnounceDate	2020-05-11
AnnouncementXML	Submission_2020-05-11_09:02:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Matthew Monroe
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Acetyl
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-22 10:53:53	ID requested
⏵ 1	2020-05-11 09:02:09	announced

Publication List

Zhou M, Uwugiaren N, Williams SM, Moore RJ, Zhao R, Goodlett D, Dapic I, Pa, š, a-Toli, ć L, Zhu Y, Sensitive Top-Down Proteomics Analysis of a Low Number of Mammalian Cells Using a Nanodroplet Sample Processing Platform. Anal Chem, 92(10):7087-7095(2020) [pubmed]

Zhou M, Uwugiaren N, Williams SM, Moore RJ, Zhao R, Goodlett D, Dapic I, Pa, š, a-Toli, ć L, Zhu Y, Sensitive Top-Down Proteomics Analysis of a Low Number of Mammalian Cells Using a Nanodroplet Sample Processing Platform. Anal Chem, 92(10):7087-7095(2020) [pubmed]

Keyword List

submitter keyword: top-down, proteomics, microfluidics, nanodroplet

submitter keyword: top-down, proteomics, microfluidics, nanodroplet

Contact List

Ying Zhu
contact affiliation	Pacific Northwest National Laboratory
contact email	ying.zhu@pnnl.gov
lab head
Matthew Monroe
contact affiliation	Pacific Northwest National Laboratory
contact email	matthew.monroe@pnnl.gov
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084612/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084612/