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PXD016399

PXD016399 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAubergine protein interactors in 0-2 hour-Drosophila embryos
DescriptionTo identify Aub function in mRNA regulation in the Drosophila embryo, we have performed mass spectrometry analysis of Aub interactors, following immunoprecipitation of GFP-Aub in 0-2 hour-embryos. Immunoprecipitation of GFP alone was used as negative control. Because Aub accumulates at high levels in the germ plasm, GFP-Aub immunoprecipitation was also performed in oskar mutant embryos that do not assemble the germ plasm. Proteins coprecipitating with GFP-Aub were similar in wild-type and oskar mutant embryos. Translation factors were enriched among proteins coprecipitating with Aub.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:02:47.848.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterOana Vigy
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-11-22 01:23:02ID requested
12020-04-27 02:57:39announced
22024-10-22 05:02:48announced2024-10-22: Updated project metadata.
Publication List
10.1038/s41422-020-0294-9;
Ramat A, Garcia-Silva MR, Jahan C, Na, ï, t-Sa, ï, di R, Dufourt J, Garret C, Chartier A, Cremaschi J, Patel V, Decourcelle M, Bastide A, Juge F, Simonelig M, The PIWI protein Aubergine recruits eIF3 to activate translation in the germ plasm. Cell Res, 30(5):421-435(2020) [pubmed]
Keyword List
submitter keyword: Aub
PIWI protein
translation
Drosophila
embryos
Contact List
Martine Simonelig
contact affiliationmRNA Regulation and Development, Institute of Human Genetics, CNRS-Univ Montpellier, Montpellier, France
contact emailMartine.Simonelig@igh.cnrs.fr
lab head
Oana Vigy
contact affiliationFPP CNRS
contact emailoana.vigy@fpp.cnrs.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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