PXD016399

PXD016399 is an original dataset announced via ProteomeXchange.

Title	Aubergine protein interactors in 0-2 hour-Drosophila embryos
Description	To identify Aub function in mRNA regulation in the Drosophila embryo, we have performed mass spectrometry analysis of Aub interactors, following immunoprecipitation of GFP-Aub in 0-2 hour-embryos. Immunoprecipitation of GFP alone was used as negative control. Because Aub accumulates at high levels in the germ plasm, GFP-Aub immunoprecipitation was also performed in oskar mutant embryos that do not assemble the germ plasm. Proteins coprecipitating with GFP-Aub were similar in wild-type and oskar mutant embryos. Translation factors were enriched among proteins coprecipitating with Aub.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:02:47.848.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oana Vigy
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-22 01:23:02	ID requested
1	2020-04-27 02:57:39	announced
⏵ 2	2024-10-22 05:02:48	announced	2024-10-22: Updated project metadata.

10.1038/s41422-020-0294-9;

Ramat A, Garcia-Silva MR, Jahan C, Na, ï, t-Sa, ï, di R, Dufourt J, Garret C, Chartier A, Cremaschi J, Patel V, Decourcelle M, Bastide A, Juge F, Simonelig M, The PIWI protein Aubergine recruits eIF3 to activate translation in the germ plasm. Cell Res, 30(5):421-435(2020) [pubmed]

submitter keyword: Aub

PIWI protein

translation

Drosophila

embryos

Martine Simonelig
contact affiliation	mRNA Regulation and Development, Institute of Human Genetics, CNRS-Univ Montpellier, Montpellier, France
contact email	Martine.Simonelig@igh.cnrs.fr
lab head
Oana Vigy
contact affiliation	FPP CNRS
contact email	oana.vigy@fpp.cnrs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD016399

PRIDE project URI

• PRIDE
  1. PXD016399
     1. Label: PRIDE project
     2. Name: Aubergine protein interactors in 0-2 hour-Drosophila embryos