PXD016398

PXD016398 is an original dataset announced via ProteomeXchange.

Title	Impact of Zika Virus on the Human Type I Interferon Osteoimmune Response
Description	Background: The developing field of osteoimmunology supports importance of an interferon(IFN) response pathway in osteoblasts. Clarifying osteoblast-IFN interactions is important because IFN is used as salvage anti-tumor therapy but systemic toxicity is high with variable clinical results. In addition, osteoblast response to systemic bursts and disruptions of IFN pathways induced by viral infection may influence bone remodeling. ZIKA virus(ZIKV) infection impacts bone development in humans and IFN response in vitro. Consistently, initial evidence of permissivity to ZIKV has been reported in human osteoblasts. Hypothesis: Osteoblast-like Saos-2 cells are permissive to ZIKV and responsive to IFN. Methods: Multiple approaches were used to assess whether Saos-2 cells are permissive to ZIKV infection and exhibit IFN-mediated ZIKV suppression. Proteomic methods were used to evaluate impact of ZIKV and IFN on Saos-2 cells. Results: Evidence is presented confirming Saos-2 cells are permissive to ZIKV and support IFN-mediated suppression of ZIKV. ZIKV and IFN differentially impact the Saos-2 proteome. Both ZIKV and IFN suppress proteins associated with microcephaly/pseudo-TORCH syndrome (BI1, KIF20 and UBP18), and ZIKV induces potential entry factor PLVAP. Conclusions: Transient ZIKV infection influences osteoimmune state, and IFN and ZIKV activate distinct proteomes in Saos-2 cells, which could inform therapeutic, engineered, disruptions.
HostingRepository	PRIDE
AnnounceDate	2020-11-03
AnnouncementXML	Submission_2020-11-02_23:10:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthew Berberich
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-22 01:22:16	ID requested
⏵ 1	2020-11-02 23:10:21	announced

Drouin A, Wallbillich N, Theberge M, Liu S, Katz J, Bellovoda K, Se Yun Cheon S, Gootkind F, Bierman E, Zavras J, Berberich MJ, Kalocsay M, Guastaldi F, Salvadori N, Troulis M, Fusco DN, Impact of Zika virus on the human type I interferon osteoimmune response. Cytokine, 137():155342(2021) [pubmed]

submitter keyword: osteoimmunology, osteoblast, interferon, interferon effector, HELZ2, Zika virus, Lumos

Dahlene N. Fusco MD PhD
contact affiliation	Assistant Professor of Medicine Department of Medicine Tulane University School of Medicine 1430 Tulane Avenue, New Orleans, LA,70114
contact email	dfusco@tulane.edu
lab head
Matthew Berberich
contact affiliation	Center for Protein Degradation/Dana-Farber Cancer Institute
contact email	matthew_berberich@dfci.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD016398

PRIDE project URI

• PRIDE
  1. PXD016398
     1. Label: PRIDE project
     2. Name: Impact of Zika Virus on the Human Type I Interferon Osteoimmune Response