PXD016395

PXD016395 is an original dataset announced via ProteomeXchange.

Title	Leaf-cutting ant feces LC-MS/MS
Description	Leaf-cutting ants of the genera Acromyrmex and Atta live in mutualistic symbiosis with a basidiomycete fungus (Leucocoprinus gongylophorus), which they cultivate as fungal gardens in underground nest chambers. The ants provide the fungus with a growth substrate consisting of freshly cut leaf fragments. After new leaf fragments are brought into the nest, the ants chew them into smaller pieces and apply droplets of fecal fluid to the leaf pulp before depositing this mixed substrate in the fungus garden and inoculating it with small tufts of mycelium from older parts of the garden. Previous work has shown that the fecal fluid contains a range of digestive enzymes including proteases, amylases, chitinases, cellulases, pectinases, hemicellulases and laccases, and that most of these enzymes are produced by the fungal symbiont in specialized structures called gongylidia that the ants eat. After ingestion, the enzymes apparently pass unharmed through the alimentary channel of the ants and end up in the fecal fluid. Most likely this complex system is an adaptation of the ant-fungus symbiosis to a herbivorous lifestyle, as the ancient ancestors of the ants and the fungus lived as hunter-gatherers and saprotrophs, respectively. The promise of fecal fluid for getting insight into the molecular adaptations that enables the ant-fungus holosymbiont to live as a herbivore, led us to investigate the fecal fluid proteome using LC-MS/MS in order to get a more comprehensive picture of the repertoire of proteins present.
HostingRepository	PRIDE
AnnounceDate	2021-01-12
AnnouncementXML	Submission_2021-01-12_00:00:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Morten Schioett
SpeciesList	scientific name: Acromyrmex echinatior; NCBI TaxID: 103372;
ModificationList	iodoacetamide derivatized residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-22 01:22:00	ID requested
⏵ 1	2021-01-12 00:00:44	announced

Dataset with its publication pending

submitter keyword: Acromyrmex, enzymes, symbiosis

Jacobus Jan Boomsma
contact affiliation	Section for Ecology and Evolution, Department of Biology, University of Copenhagen, Denmark
contact email	jjboomsma@bio.ku.dk
lab head
Morten Schioett
contact affiliation	DTU
contact email	mosch@aqua.dtu.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016395
     1. Label: PRIDE project
     2. Name: Leaf-cutting ant feces LC-MS/MS