PXD016385

PXD016385 is an original dataset announced via ProteomeXchange.

Title	Proteins associated with RORalpha binding element on SULT1E1 gene
Description	Estrogen sulfotransferase (SULT1E1) metabolically inactivates estrogen and SULT1E1 expression is tightly regulated by multiple nuclear receptors in cells. A series of biochemical techniques have clearly demonstrated that nuclear receptors HNF4alpha and phosphorylated RORalpha at Ser100 form as complexes binding to the SULT1E1 enhancer to activate gene transcription in response to a high glucose (450 mg/dL) signal. However, the kinase that mediates this phosphorylation at high glucose condition remain in mysterious. To identify these binding factors and possible kinases that mediate the phosphorylation on the SULT1E1 enhancer, the SULT1E1 enhancer was biotinylated and conjugated to magnetic beads, which were subsequently used to enrich the binding factors from low glucose (40 mg/dL) and high glucose (450 mg/dL) treated HepG2 cell nuclear extracts.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:11:20.213.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jason Williams
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-21 14:06:04	ID requested
1	2020-08-26 07:48:47	announced
⏵ 2	2024-10-22 05:11:21	announced	2024-10-22: Updated project metadata.

Hu H, Negishi M, as glucose signal to regulate estrogen sulfation in human liver cells. Biochem J, 477(18):3583-3598(2020) [pubmed]

10.1042/bcj20200427;

submitter keyword: AP-MS, SULT1E1 gene,LC-MSMS, RORalpha binding element

Jason Williams
contact affiliation	Mass Spectrometry Research and Support Group National Institute of Environmental Health Sciences
contact email	willia56@niehs.nih.gov
lab head
Jason Williams
contact affiliation	National Institute of Environmental Health Sciences
contact email	willia56@niehs.nih.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016385
     1. Label: PRIDE project
     2. Name: Proteins associated with RORalpha binding element on SULT1E1 gene