PXD016357

PXD016357 is an original dataset announced via ProteomeXchange.

Title	Identification of ELC interacting proteins in zebrafish heart
Description	The essential myosin light chain (ELC) is part of the cardiac myosin motor regulating the contractility of the heart. Recently, we have demonstrated ELC phosphorylation to be responsible not only for basal maintenance of cardiac contractility, but also for the adaptation of sarcomere function to increased demands in adult zebrafish (Scheid et al. Cardiovascular Research, 2016). However, the direct interacting kinases, phosphatases as well as the signaling pathway mediating the ELC phosphorylation are mostly unknown. Here, we aim to identify ELC interacting proteins in zebrafish heart tissue using an ELC-specific precipitation assay followed by mass spectrometry. In brief, ELC specific co-immunoprecipitation was performed by using the Dynabeads Co-immunoprecipitation Kit (Invitrogen, #14321D). Custom-specific polyclonal ELC antibodies (Eurogentec, peptide for immunization: NH2-CAPVPETPKEPEVDLK-CONH2) against zebrafish ELC were covalently coupled to DynabeadsTM M-270 Epoxy (Invitrogen, #14302D) and whole zebrafish heart protein was used as input. All steps were carried out according to the manufacturer's instructions.
HostingRepository	PRIDE
AnnounceDate	2022-07-24
AnnouncementXML	Submission_2022-07-24_02:31:44.709.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Ruppert
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-20 01:16:55	ID requested
⏵ 1	2022-07-24 02:31:45	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: myosin light chain, ELC, phosphorylation, co-immunoprecipitation

Thomas Ruppert
contact affiliation	Centre of Molecular Biology University Heidelberg Im Neuenheimer Feld 282 D-69120 Heidelberg Germany
contact email	t.ruppert@zmbh.uni-heidelberg.de
lab head
Thomas Ruppert
contact affiliation	ZMBH, Im Neuenheimer Feld 282, 69122 Heidelberg
contact email	t.ruppert@zmbh.uni-heidelberg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016357
     1. Label: PRIDE project
     2. Name: Identification of ELC interacting proteins in zebrafish heart