PXD016347

PXD016347 is an original dataset announced via ProteomeXchange.

Title	TBP2/TFIIA complex drives an oocyte-type promoter usage grammar leading to female germ cell specific mRNA and endogenous retroviral element transcription
Description	The transcriptional machinery involved in RNA polymerase II (Pol II) transcription during oogenesis is not well characterized. In somatic cells, the Pol II general transcription factor, TFIID, containing the TATA binding protein (TBP) and 13 TBP-associated factors, is the first to bind gene promoters to nucleate pre-initiation complex formation. During oocyte growth TBP is replaced by TBP2 and Tbp2-/- female mice are sterile. Here, we show that in oocytes TBP2 stably associates with TFIIA but does not assemble into a TFIID-type complex but stabily associates with TFIIA. we demonstrate that in female germ cells the specific TBP2-TFIIA-containing transcription machinery drives transcription from oocyte-specific core promoters, which are different from those occupied by TBP/TFIID in somatic cells.
HostingRepository	PRIDE
AnnounceDate	2020-11-16
AnnouncementXML	Submission_2020-11-16_08:05:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	STEPHANE VINCENT
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-19 06:47:49	ID requested
⏵ 1	2020-11-16 08:05:19	announced

Dataset with its publication pending

submitter keyword: Mouse, growing oocyte, TBPL2, TFIIA

Laszlo Tora
contact affiliation	1-Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch, France; 2-Centre National de la Recherche Scientifique (CNRS), UMR7104, 67404 Illkirch, France; 3-Institut National de la Santé et de la Recherche Médicale (INSERM), U1258, 67404 Illkirch, France 4-Université de Strasbourg, 67404 Illkirch, France
contact email	lazslo@igbmc.fr
lab head
STEPHANE VINCENT
contact affiliation	INSERM IGBMC
contact email	VINCENT@IGBMC.FR
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD016347

PRIDE project URI

• PRIDE
  1. PXD016347
     1. Label: PRIDE project
     2. Name: TBP2/TFIIA complex drives an oocyte-type promoter usage grammar leading to female germ cell specific mRNA and endogenous retroviral element transcription