PXD016346

PXD016346 is an original dataset announced via ProteomeXchange.

Title	Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosis
Description	Nanohaloarchaeota, a clade of diminutive archaea, with small genomes and limited metabolic capabilities, are ubiquitous in hypersaline habitats, which they share with the extremely halophilic and phylogenetically distant euryarchaea. Some of these nanohaloarchaeota and euryarchaea appear to interact with each other. In this study, we investigate the genetic and physiological nature of their relationship. We isolated the nanohaloarchaeon Candidatus Nanohalobium constans LC1Nh and the haloarchaeon Halomicrobium sp. LC1Hm from a solar saltern, reproducibly co-cultivated these species, sequenced their genomes, and characterized their metabolic/trophic interactions. The nanohaloarchaeon is a magnesium-dependent aerotolerant heterotrophic anaerobe of the DPANN superphylum; it lacks respiratory complexes and its energy production relies on fermentative metabolism of sugar derivatives, obtained by depolymerizing alpha-glucans or by acquiring the chitin monomer N-acetylglucosamine from the chitinolytic haloarchaeal host. Halomicrobium is a member of the class Halobacteria and a chitinotrophic aerobe. The nanohaloarchaeon lacks key biosynthetic pathways and is likely to be provided with amino acids, lipids, nucleotides and cofactors via physical contact with its host Halomicrobium. In turn, the ability of Ca. Nanohalobium to hydrolyse alpha-glucans boosts the host’s growth in the absence of a chitin substrate. These findings suggest that at least some members of the nanohaloarchaea, previously considered ecologically unimportant given their limited metabolic potential, in fact may play significant roles in the microbial carbon turnover, food chains, and ecosystem function. The behaviour of Halomicrobium, which accommodates the colonization by Ca. Nanohalobium, can be interpreted as a bet-hedging strategy, maximizing its long-term fitness in a habitat where the availability of carbon substrates can vary both spatially and temporarily.
HostingRepository	PRIDE
AnnounceDate	2020-07-24
AnnouncementXML	Submission_2020-08-11_22:46:38.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD016346
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sergio Ciordia
SpeciesList	scientific name: environmental samples ; NCBI TaxID: 1462472;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-19 06:47:11	ID requested
1	2020-07-23 23:20:18	announced
⏵ 2	2020-08-11 22:46:39	announced	2020-08-12: Updated publication reference for PubMed record(s): 32759215.

La Cono V, Messina E, Rohde M, Arcadi E, Ciordia S, Crisafi F, Denaro R, Ferrer M, Giuliano L, Golyshin PN, Golyshina OV, Hallsworth JE, La Spada G, Mena MC, Merkel AY, Shevchenko MA, Smedile F, Sorokin DY, Toshchakov SV, Yakimov MM, Symbiosis between nanohaloarchaeon and haloarchaeon is based on utilization of different polysaccharides. Proc Natl Acad Sci U S A, 117(33):20223-20234(2020) [pubmed]

submitter keyword: Haloarchaea, Hypersaline solar saltern, Degradation of chitin, Nanohaloarchaea, DPANN superphilum

Michail M. Yakimov
contact affiliation	Institute for Biological Resources and Marine Biotechnologies (CNR), Italian National Research Council, Messina, Italy
contact email	mikhail.iakimov@cnr.it
lab head
Sergio Ciordia
contact affiliation	Spanish National Center for Biotechnology
contact email	sciordia@cnb.csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD016346

PRIDE project URI

• PRIDE
  1. PXD016346
     1. Label: PRIDE project
     2. Name: Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosis