PXD016342-1
PXD016342 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mass spectrometry of in-gel digests reveals differences in amino acid sequences of high-molecular-weight glutenin subunits in spelt and emmer compared to common wheat |
Description | High-molecular-weight glutenin subunits (HMW-GS) play an important role for the baking quality of wheat. The ancient wheats emmer and spelt differ in their HMW-GS pattern compared to modern common wheat and this might be one reason for their comparatively poor baking quality. The aim of this study was to elucidate similarities and differences in the amino acid sequences of two 1Bx HMW-GS of common wheat, spelt and emmer. First, the sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE) system was optimized to separate common wheat, spelt and emmer Bx6 and Bx7 from other HMW-GS (e.g., 1Ax and 1By) in high concentrations. The in-gel digests of the Bx6 and Bx7 bands were analyzed by untargeted LC-MS/MS experiments revealing different UniProtKB accessions in spelt and emmer compared to common wheat. The HMW-GS Bx6 and Bx7, respectively, of emmer and spelt showed differences in the amino acid sequences compared to those of common wheat. The identities of the peptide variations were confirmed by targeted LC-MS/MS. These peptides can be used to differentiate between Bx6 and Bx7 of spelt and emmer and Bx6 and Bx7 of common wheat. The findings should help to increase the reliability and curation status of wheat protein databases and to understand the effects of protein structure on the functional properties. |
HostingRepository | PanoramaPublic |
AnnounceDate | 2020-01-15 |
AnnouncementXML | Submission_2020-01-15_12:16:51.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Sabrina Geisslitz |
SpeciesList | scientific name: Triticum dicoccoides; NCBI TaxID: 85692; scientific name: Triticum aestivum; NCBI TaxID: 4565; scientific name: Triticum spelta; NCBI TaxID: 58933; |
ModificationList | No PTMs are included in the dataset |
Instrument | QTRAP 6500+ |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-11-19 06:37:19 | ID requested | |
⏵ 1 | 2020-01-15 12:16:53 | announced |
Publication List
Geisslitz S, America AHP, Scherf KA, Mass spectrometry of in-gel digests reveals differences in amino acid sequences of high-molecular-weight glutenin subunits in spelt and emmer compared to common wheat. Anal Bioanal Chem, 412(6):1277-1289(2020) [pubmed] |
Keyword List
submitter keyword: High-molecular weight glutenin subunits, spelt, emmer, einkorn, LC-MS/MS, SDS-PAGE |
Contact List
Katharina Scherf | |
---|---|
contact affiliation | Department of Bioactive and Functional Food Chemistry, Institute of Applied Biosciences, Karlsruhe Institute of Technology |
contact email | k.scherf.leibniz-lsb@tum.de |
lab head | |
Sabrina Geisslitz | |
contact affiliation | Leibniz-Institute for Food Systems Biology at the Technical University of Munich |
contact email | s.geisslitz.leibniz-lsb@tum.de |
dataset submitter |
Full Dataset Link List
Panorama Public dataset URI |