PXD016337

PXD016337 is an original dataset announced via ProteomeXchange.

Title	Tetraponera aethiops venom peptidome
Description	In the mutualisms involving certain pseudomyrmicine ants and different myrmecophytes (i.e., plants sheltering colonies of specialized ���������plant-ant��������� species in hollow structures), the ant venom contributes to the host plant biotic defenses by inducing the rapid paralysis of defoliating insects and causing intense pain to browsing mammals. Using integrated transcriptomic and proteomic approaches, we identified the venom peptidome of the plant-ant Tetraponera aethiops (Pseudomyrmecinae). The transcriptomic analysis of its venom glands revealed that 40 % of the assembled contigs encoded only seven peptide precursors related to the ant venom peptides from the A-superfamily. Among the 12 peptide masses detected by liquid chromatography-mass spectrometry (LC-MS), nine mature peptide sequences were characterized and confirmed through proteomic analysis. These venom peptides, called pseudomyrmecitoxins (PSDTX), share amino acid sequence homologies with myrmeciitoxins known for their dual offensive and defensive functions on both insects and mammals. Furthermore, we demonstrated through reduction/alkylation of the crude venom that four PSDTXs were homo- and heterodimeric. Thus, we provide the first indication that the defensive venom composition of the ant genus Tetraponera is a streamlined peptidome. We provide the first insights into the defensive venom composition of the ant genus Tetraponera indicative of a streamlined peptidome.
HostingRepository	PRIDE
AnnounceDate	2020-01-10
AnnouncementXML	Submission_2020-01-10_05:49:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Axel Touchard
SpeciesList	scientific name: Tetraponera aethiops; NCBI TaxID: 219813;
ModificationList	amidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-19 04:10:04	ID requested
⏵ 1	2020-01-10 05:49:25	announced

Barass, é V, Touchard A, T, é, n, é N, Tindo M, Kenne M, Klopp C, Dejean A, Bonnaf, é E, Treilhou M, (Formicidae: Pseudomyrmecinae). Toxins (Basel), 11(12):(2019) [pubmed]

submitter keyword: ant, venom, peptide, dimeric toxin

Treilhou Michel
contact affiliation	EA-7417, Institut National Universitaire Champollion, Place de Verdun, 81012 Albi, France
contact email	michel.treilhou@univ-jfc.fr
lab head
Axel Touchard
contact affiliation	Institut National Universitaire de Champollion
contact email	t.axel@hotmail.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016337
     1. Label: PRIDE project
     2. Name: Tetraponera aethiops venom peptidome