PXD016334

PXD016334 is an original dataset announced via ProteomeXchange.

Title	Cross-regulation of viral kinases with cyclin A secures shutoff of host DNA synthesis
Description	Herpesviruses encode conserved protein kinases to optimize virus infection by targeted phosphorylation. How these kinases bind to cellular factors and how this impacts their regulatory functions is poorly understood. Here, we use quantitative proteomics to determine the interactomes of eight herpesvirus kinases. We identify Cyclin A binding as a distinguishing feature of betaherpesvirus kinases that were previously described as viral mimics of cyclin-dependent kinases (v-CDKs), inert to cellular control. RXL motifs within the non-catalytic regions of roseolo- and muromegalovirus v-CDKs serve as Cyclin A docking sites and closely overlap with nuclear localization signals (NLS). By competing with NLS function, Cyclin A binding redirects NLS-RXL containing v-CDKs to cytoplasmic substrates at late times of infection. Concomitantly, Cyclin A is sequestered to the cytoplasm, which is essential for the viral inhibition of cellular DNA replication. Our data highlight a fine-tuned and physiologically important interplay between a cellular cyclin and viral CDK-like kinases.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:10:47.140.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Boris Bogdanow
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Herpesvirales; NCBI TaxID: NCBITaxon:548681;
ModificationList	phosphorylated residue; deaminated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-19 02:15:06	ID requested
1	2020-08-12 22:31:17	announced
2	2020-10-19 01:53:37	announced	2020-10-19: Updated publication reference for PubMed record(s): 32973148.
⏵ 3	2024-10-22 05:10:49	announced	2024-10-22: Updated project metadata.

10.1038/s41467-020-18542-1;

Bogdanow B, Schmidt M, Weisbach H, Gruska I, Vetter B, Imami K, Ostermann E, Brune W, Selbach M, Hagemeier C, Wiebusch L, Cross-regulation of viral kinases with cyclin A secures shutoff of host DNA synthesis. Nat Commun, 11(1):4845(2020) [pubmed]

submitter keyword: HCMV, EBV,conserved herpesvirus protein kinases, HSV-1, MCMV

Matthias Selbach
contact affiliation	MDC Berlin
contact email	matthias.selbach@mdc-berlin.de
lab head
Boris Bogdanow
contact affiliation	MDC Berlin
contact email	boris.bogdanow@mdc-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD016334

PRIDE project URI

• PRIDE
  1. PXD016334
     1. Label: PRIDE project
     2. Name: Cross-regulation of viral kinases with cyclin A secures shutoff of host DNA synthesis