PXD016325

PXD016325 is an original dataset announced via ProteomeXchange.

Title	Integrating serum protein electrophoresis with mass spectrometry, a new workflow for M-protein detection and quantification
Description	Serum protein electrophoresis (SPE) and immunofixation electrophoresis (IFE) are standard tools for multiple myeloma (MM) routine diagnostics. M-protein is a biomarker for MM that can be quantified with SPE and characterized with IFE. We have investigated combining SPE/IFE with targeted mass spectrometry (MS) to detect and quantify the M-protein. SPE-MS assay offers the possibility to detect M-protein with higher sensitivity than SPE/IFE, which could lead to better analysis of minimal residual disease in clinical laboratories. In addition, analysis of archived SPE gels could be used for retrospective MM studies. We have investigated two different approaches of measuring M-protein and therapeutic monoclonal antibodies (t-mAbs) from SPE/IFE gels. After extracting proteotypic peptides from the gel, they can be quantified using stable isotope labeled (SIL) peptides and measured by Orbitrap mass spectrometry. Alternatively, extracted peptides can be labeled with tandem mass tags (TMT). Both approaches are not hampered by the presence of t-mAbs. Using SIL peptides, limit of detection of the M-protein is approximately 100-fold better than with routine SPE/IFE. Using TMT labeling, M-protein can be measured and compared in different samples from the same patient. We have successfully measured M-protein proteotypic peptides extracted from the SPE/IFE gels utilizing SIL peptides and TMT.
HostingRepository	PRIDE
AnnounceDate	2020-01-07
AnnouncementXML	Submission_2020-01-07_08:28:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marina Zajec
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-18 08:45:34	ID requested
⏵ 1	2020-01-07 08:28:53	announced

Zajec M, Jacobs JFM, de Kat Angelino CM, Dekker LJM, Stingl C, Luider TM, De Rijke YB, VanDuijn MM, Integrating Serum Protein Electrophoresis with Mass Spectrometry, A New Workflow for M-Protein Detection and Quantification. J Proteome Res, 19(7):2845-2853(2020) [pubmed]

submitter keyword: serum protein electrophoresis, immunofixation electrophoresis, targeted mass spectrometry, multiple myeloma, M-protein

Theo Luider
contact affiliation	Department of Neurology, Erasmus MC, Rotterdam, The Netherlands
contact email	t.luider@erasmusmc.nl
lab head
Marina Zajec
contact affiliation	Erasmus MC
contact email	m.zajec@erasmusmc.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016325
     1. Label: PRIDE project
     2. Name: Integrating serum protein electrophoresis with mass spectrometry, a new workflow for M-protein detection and quantification