PXD016322

PXD016322 is an original dataset announced via ProteomeXchange.

Title	Human bone marrow comprise atypical adipocytes with specific lipid metabolism
Description	Under caloric restriction, bone marrow adipocytes (BM-Ad) do not decrease in size conversely to white adipocytes, suggesting their unique metabolic properties. We compared human primary BM-Ad with paired subcutaneous adipocytes (SC-Ad) using proteomic and lipidomic approaches. We found that while SC-Ad and BM-Ad share similar morphological features, they possess distinct lipid metabolism. BM-Ad shows enrichment in proteins involved in cholesterol metabolism correlating with increased free cholesterol content while proteins involved in lipolysis were downregulated. In particular, monoacylglycerol lipase expression was strongly reduced in BM-Ad, leading to accumulation of monoacylglycerol. Consequently, basal and induced lipolytic responses were absent in BM-Ad, affirming their differences in metabolic fitness upon caloric restriction. These specific metabolic features are not recapitulated in vitro using common protocols to differentiate bone marrow mesenchymal stem cells. Thus, contrary to classical SC-Ad, BM-Ad display a specific lipid metabolism, as they are devoid of lipolytic activity and exhibit a cholesterol-orientated metabolism.
HostingRepository	PRIDE
AnnounceDate	2020-01-29
AnnouncementXML	Submission_2020-02-17_03:23:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manuelle Ducoux-Petit
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-18 07:32:58	ID requested
1	2020-01-29 03:53:05	announced
⏵ 2	2020-02-17 03:23:10	announced	2020-02-17: Updated publication reference for PubMed record(s): 31995765.

Attan, é C, Est, è, ve D, Chaoui K, Iacovoni JS, Corre J, Moutahir M, Valet P, Schiltz O, Reina N, Muller C, Human Bone Marrow Is Comprised of Adipocytes with Specific Lipid Metabolism. Cell Rep, 30(4):949-958.e6(2020) [pubmed]

submitter keyword: Bone Marrow adipocytes, human, label free quantificatio, LC-MSMS

Catherine Muller
contact affiliation	Team: Microenvironnement, Cancer and Adipocytes Institute of Pharmacology and Structural Biology BP64182 205 route de Narbonne 31077 Toulouse Cedex04 France
contact email	muller@ipbs.fr
lab head
Manuelle Ducoux-Petit
contact affiliation	CNRS
contact email	manuelle.ducoux@ipbs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD016322

PRIDE project URI

• PRIDE
  1. PXD016322
     1. Label: PRIDE project
     2. Name: Human bone marrow comprise atypical adipocytes with specific lipid metabolism