PXD016321

PXD016321 is an original dataset announced via ProteomeXchange.

Title	Mitochondrial Proteome of Affected Neurons in a Mouse Model of Leigh Syndrome
Description	Defects in mitochondrial function lead to severe neuromuscular orphan pathologies known as mitochondrial disease. Among them, Leigh Syndrome is the most common pediatric presentation, characterized by symmetrical brain lesions, hypotonia, motor and respiratory deficits, and premature death. Mitochondrial diseases are characterized by a marked anatomical and cellular specificity. However, the molecular determinants for this susceptibility are currently unknown, hindering the efforts to find an effective treatment. Due to the complex crosstalk between mitochondria and their supporting cell, strategies to assess the underlying alterations in affected cell types in the context of mitochondrial dysfunction are critical. Here, we developed a novel virus-based tool, the AAV-mitoTag viral vector, to isolate mitochondria from genetically-defined cell types. Administration of the AAV-mitoTag in the vestibular neurons of a mouse model of Leigh Syndrome lacking the complex I subunit Ndufs4 allowed us to assess the proteome and acetylome of susceptible neurons in a well characterized model recapitulating the human disease. Our results show a marked reduction of complex-I N-module subunit abundance and an increase in the levels of the assembly factor NDUFA2. Transiently-associated non-mitochondrial proteins such as PKCδ, and the complement subcomponent C1Q were also increased in Ndufs4-deficient mitochondria. Furthermore, lack of Ndufs4 induced pyruvate dehydrogenase (PDH) subunit hyperacetylation, leading to decreased PDH activity. We provide novel insight on the pathways involved in mitochondrial disease, which could underlie potential therapeutic approaches for these pathologies.
HostingRepository	PRIDE
AnnounceDate	2020-07-28
AnnouncementXML	Submission_2020-07-28_05:03:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alex Gella
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-18 07:30:54	ID requested
⏵ 1	2020-07-28 05:03:44	announced

Bolea I, Gella A, Sanz E, Prada-Dacasa P, Menardy F, Bard AM, Machuca-M, á, rquez P, Eraso-Pichot A, M, ò, dol-Caballero G, Navarro X, Kalume F, Quintana A, Defined neuronal populations drive fatal phenotype in a mouse model of Leigh syndrome. Elife, 8():(2019) [pubmed]

submitter keyword: Leigh Syndrome, animal models, neuroscience, proteomics, cell type-specific, mitochondrial isolation

Albert Quintana
contact affiliation	1) 1Mitochondrial Neuropathology laboratory, Institut de Neurociències, Universitat Autònoma de Barcelona, Bellaterra, Spain 2) Department of Cellular Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, Bellaterra, Spain.
contact email	albert.quintana@uab.cat
lab head
Alex Gella
contact affiliation	Autonomous University of Barcelona
contact email	Alex.Gella@uab.cat
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016321
     1. Label: PRIDE project
     2. Name: Mitochondrial Proteome of Affected Neurons in a Mouse Model of Leigh Syndrome