PXD016316

PXD016316 is an original dataset announced via ProteomeXchange.

Title	Transgelin silencing induces different processes in differrent breast cancer cell lines
Description	Transgelin is a protein which has been described as a marker of several cancers. Interestingly, the literature describes both up- and down-regulation of transgelin in the tumor in comparison with non-tumor tissue. The mechanisms of transgelin function in cancer are considerably unknown. Transgelin is abundant especially in smooth muscle cells. It is associated with actin stress fibers. These contractile structures participate among others in cell motility, adhesion or morphology. We focused on transgelin function in breast cancer. Initially, we studied transgelin role in cell migration in the breast cancer cell lines BT 549 and PMC 42. Using xCELLigence system we reached opposite results in two cell lines. Transgelin silencing increased and decreased migration of PMC 42 and BT 549 cells, respectively. To further clarify these contradictory results, we performed an experiment to quantify proteomic changes after transgelin silencing in these two cell lines using quantitative proteomics (iTRAQ-2DLC-MS/MS). Our results confirmed transgelin function in the migration of BT 549 cells and suggested transgelin role in apoptosis and small molecule biochemistry in PMC 42 cells.
HostingRepository	PRIDE
AnnounceDate	2020-02-21
AnnouncementXML	Submission_2020-02-21_01:38:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pavel Bouchal
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-18 01:42:09	ID requested
⏵ 1	2020-02-21 01:38:11	announced

Dvorakova M, Lapcik P, Bouchalova P, Bouchal P, Transgelin Silencing Induces Different Processes in Different Breast Cancer Cell Lines. Proteomics, 20(7):e1900383(2020) [pubmed]

submitter keyword: Transgelin

breast cancer

metastasis

migration

apoptosis

cytoskeleton

Pavel Bouchal
contact affiliation	Masaryk University, Faculty of Science
contact email	bouchal@chemi.muni.cz
lab head
Pavel Bouchal
contact affiliation	Masaryk University, Faculty of Science
contact email	bouchal@chemi.muni.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016316
     1. Label: PRIDE project
     2. Name: Transgelin silencing induces different processes in differrent breast cancer cell lines