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PXD016304

PXD016304 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTowards the molecular architecture of the peroxisomal receptor docking complex
DescriptionImport of yeast peroxisomal matrix proteins is initiated by cytosolic receptors, which specifically recognize and bind the respective cargo proteins. At the peroxisomal membrane, the cargo-loaded receptor interacts with the membrane docking complex, composed of Pex14p, Pex17p and Pex13p. Previous data suggest that this interaction triggers the formation of an import pore for further translocation of the cargo. The mechanistic principles and the underlying mechanism are however unclear, mainly because structures of higher order assemblies are still lacking. Here, using an integrative approach, we provide the first structural characterization of the major components of the peroxisomal docking complex Pex14p/Pex17p, in a native bilayer environment and reveal its subunit organization. Our data show that three copies of Pex14p and a single copy of Pex17p assemble to form a 20 nm rod-like particle. The different subunits are arranged in a parallel manner, showing interactions along their complete sequences and providing receptor binding-sites on both membrane sides. The long rod facing the cytosol is mainly formed by the coiled-coil domains of Pex14p and Pex17p, possibly providing the necessary structural support for the formation of the import pore. Further implications of Pex14p/Pex17p for formation of the peroxisomal translocon are discussed.
HostingRepositoryPRIDE
AnnounceDate2020-12-16
AnnouncementXMLSubmission_2020-12-16_03:54:09.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFriedel Drepper
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListmonohydroxylated residue
InstrumentQ Exactive; LTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-11-18 00:22:13ID requested
12020-12-16 03:54:09announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: yeast, crosslinking, LC-MS, structural MS, native MS
Contact List
Bettina Warscheid
contact affiliationFunctional Proteomics Lab, Faculty of Biology, University of Freiburg, Germany
contact emailbettina.warscheid@biologie.uni-freiburg.de
lab head
Friedel Drepper
contact affiliationAG Warscheid Biologie II Albert-Ludwigs-Universität Freiburg Schänzlestr. 1 79104 Freiburg Germany
contact emailfriedel.drepper@biologie.uni-freiburg.de
dataset submitter
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