PXD016289

PXD016289 is an original dataset announced via ProteomeXchange.

Title	Unraveling the complexities of mitochondrial supercomplexes by mass spectrometry-based proteomics
Description	It is now accepted that mitochondrial respiratory complex subunits assemble in supercomplexes. However, studying supercomplexes has typically relied upon antibody-based protein quantification, often limited to a single subunit per respiratory complex. To provide a deeper insight into mitochondrial plasticity and supercomplex formation, we combined Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) and high-resolution mass spectrometry-based proteomics in sedentary and exercise-trained skeletal muscle. We quantified 422 mitochondrial proteins within ten bands. Our analyses revealed the often-unaccounted presence of complex II and V subunits within the majority of supercomplexes. Upon exercise-induced mitochondrial biogenesis, non-stoichiometric changes in subunits and their incorporation into supercomplexes were apparent. Finally, we uncovered the dynamics of supercomplex-related assembly proteins and mtDNA-encoded subunits within supercomplexes, as well as the high-molecular mass complexes of ubiquinone biosynthesis enzymes and Lactb, a mitochondrial-localized obesogenic polymer. Thus, combining BN-PAGE and mass spectrometry to comprehensively analyze respiratory supercomplexes illuminates previously undetectable complexity in mitochondrial plasticity.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:21:56.726.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	atul shahaji deshmukh
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-15 01:37:47	ID requested
1	2021-05-16 23:03:59	announced
⏵ 2	2024-10-22 05:21:57	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: oxidative phosphorylation, mitochondrial supercomplexes, blue native gel,mitochondrial respiratory complexes, protein complex proteomics

Atul S Deshmukh
contact affiliation	Novo Nordisk Center For Basic Metabolic Research, Department of Health and Medical Sciences, Copenhagen University, Copenhagen, Denmark
contact email	atul.deshmukh@sund.ku.dk
lab head
atul shahaji deshmukh
contact affiliation	Copenhagen University
contact email	atul.deshmukh@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD016289

PRIDE project URI

• PRIDE
  1. PXD016289
     1. Label: PRIDE project
     2. Name: Unraveling the complexities of mitochondrial supercomplexes by mass spectrometry-based proteomics