PXD016265

PXD016265 is an original dataset announced via ProteomeXchange.

Title	Intracellular peptides mediate phenotypic differences between wild type and thimet oligopeptidase null mice possibly through their binding to microRNAs
Description	The constant increase in the obese and overweight population creates opportunities for novel therapeutic innovations to allow people to eat without getting fat and getting sick. Here, fed a hyperlipidic diet (HD)C57BL6 thimet oligopeptidase null (THOP1-/-) male mice were shown to gain only 25% (females gained 60%) of the weight gained by control wild type (WT) mice. Distinct from WT mice,THOP1-/- fed HD exhibited normal blood glucose levels and no insulin resistance. THOP1-/-fed HD phenotype benefits also include regular blood cholesterol levels, reduced fat in adipose and liver tissues, increased adipose tissue adrenergic-stimulated lipolysis, and greater resistance and endurance on treadmill running tests at high intensity. THOP1-/-compared to WT have alterations on the expression levels of specific genes and microRNAs related to obesity. Intracellular peptides that had their relative levels altered in THOP1-/-were shown to modulate the expression levels of genes and mature microRNAs associated to obesity, in 3T3L1 adipocyte. The ubiquitous existence of intracellular peptides in different cells of different species suggests a broader biological significance of the present results, connecting proteasomal protein degradation to protein synthesis. These exciting results suggest THOP1 and intracellular peptides as novel therapeutic targets to control obesity and obesity-associated diseases.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:32:36.890.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD016265
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Amanda Miquilini Cordibello
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Dimethyl:2H(6)13C(2); Oxidation; Dimethyl; Acetyl; Dimethyl:2H(4)
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-13 09:07:15	ID requested
⏵ 1	2024-10-07 10:32:37	announced

10.3390/biom10020321;

10.6019/PXD016265;

Gewehr MCF, Teixeira AAS, Santos BAC, Biondo LA, Gozzo FC, Cordibello AM, Eichler RAS, Reckziegel P, Da Silva RNO, Dos Santos NB, Camara NOS, Castoldi A, Barreto-Chaves MLM, Dale CS, Senger N, Lima JDCC, Seelaender MCL, Inada AC, Akamine EH, Castro LM, Rodrigues AC, Neto JCR, Ferro ES, The Relevance of Thimet Oligopeptidase in the Regulation of Energy Metabolism and Diet-Induced Obesity. Biomolecules, 10(2):(2020) [pubmed]

submitter keyword: obesity

insulin resistance

steatosis

overweight

proteasome

proteases

peptidases

microRNA

gene expression

mass spectrometry

peptidome

Emer Suavinho Ferro
contact affiliation	Department of Pharmacology, University of São Paulo. São Paulo, SP, Brazil.
contact email	eferro@usp.br
lab head
Amanda Miquilini Cordibello
contact affiliation	Unicamp
contact email	a230037@dac.unicamp.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/02/PXD016265

PRIDE project URI

• PRIDE
  1. PXD016265
     1. Label: PRIDE project
     2. Name: Intracellular peptides mediate phenotypic differences between wild type and thimet oligopeptidase null mice possibly through their binding to microRNAs