PXD016256

PXD016256 is an original dataset announced via ProteomeXchange.

Title	Cooperation of the NEIL3 and Fanconi Anemia/BRCA Pathways in Interstrand Crosslink Repair
Description	The NEIL3 DNA glycosylase is a base excision repair enzyme that excises bulky base lesions from DNA. Although NEIL3 has been shown to unhook interstrand crosslinks (ICL) in Xenopus extracts, how NEIL3 participants in ICL repair in human cells and its corporation with the canonical Fanconi anemia (FA)/BRCA pathway remain unclear. Here we show that the NEIL3 and the FA/BRCA pathways are non-epistatic in psoralen-ICL repair. The NEIL3 pathway is the major pathway for repairing psoralen-ICL, and the FA/BRCA pathway is only activated when NEIL3 is not present. Mechanistically, NEIL3 is recruited to psoralen-ICL in a rapid, PARP-dependent manner. Importantly, the NEIL3 pathway repairs psoralen-ICLs without generating double-strand breaks (DSBs), unlike the FA/BRCA pathway. In addition, we found that the RUVBL1/2 complex physically interact with NEIL3 and function within the NEIL3 pathway in psoralen-ICL repair. Moreover, TRAIP is important for the recruitment of NEIL3 but not FANCD2, and knockdown of TRAIP promotes FA/BRCA pathway activation. Interestingly, TRAIP is non-epistatic with both NEIL3 and FA pathways in psoralen-ICL repair, suggesting that TRAIP may function upstream of the two pathways. Taken together, the NEIL3 pathway is the major pathway to repair psoralen-ICL through a unique DSB-free mechanism in human cells.
HostingRepository	PRIDE
AnnounceDate	2020-01-20
AnnouncementXML	Submission_2020-02-17_03:20:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jia Zhou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-13 01:24:52	ID requested
1	2020-01-20 00:58:04	announced
2	2020-01-29 05:40:03	announced	2020-01-29: Updated publication reference for PubMed record(s): 31980815.
⏵ 3	2020-02-17 03:20:53	announced	2020-01-29: Updated publication reference for PubMed record(s): 31980815. 2020-02-17: Updated publication reference for PubMed record(s): 31980815.

Li N, Wang J, Wallace SS, Chen J, Zhou J, D'Andrea AD, Cooperation of the NEIL3 and Fanconi anemia/BRCA pathways in interstrand crosslink repair. Nucleic Acids Res, 48(6):3014-3028(2020) [pubmed]

submitter keyword: NEIL3, Fanconi Anemia, Psoralen Interstrand crosslink

Alan D'Andrea
contact affiliation	Dana-Farber Cancer Institute Harvard Medical School
contact email	Alan_Dandrea@dfci.harvard.edu
lab head
Jia Zhou
contact affiliation	Dana-Farber Cancer Institute
contact email	jia_zhou@dfci.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD016256

PRIDE project URI

• PRIDE
  1. PXD016256
     1. Label: PRIDE project
     2. Name: Cooperation of the NEIL3 and Fanconi Anemia/BRCA Pathways in Interstrand Crosslink Repair