PXD016249

PXD016249 is an original dataset announced via ProteomeXchange.

Title	Tracking drug action on the cell surface proteome
Description	Endogenous ligands and drugs interact with the cell-surface proteome but hypothesis-free tracking of dynamic remodeling processes at the plasma membrane is challenging. We introduce cell surface thermal proteome profiling for comprehensive characterization of ligand-induced changes in protein abundances and thermal stabilities. We demonstrate drug binding to extracellular receptors, complexes and transporters, discover stimulation-dependent remodeling of T-cell receptor complexes and describe a competition-based approach to measure target engagement of GPCR antagonists.
HostingRepository	PRIDE
AnnounceDate	2020-10-26
AnnouncementXML	Submission_2020-10-26_00:54:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mathias Kalxdorf
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-12 04:21:03	ID requested
⏵ 1	2020-10-26 00:54:51	announced

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: Thermal proteome profiling, cell surface, plasma membrane

Marcus Bantscheff
contact affiliation	Cellzome GmbH, a GSK company
contact email	marcus.x.bantscheff@gsk.com
lab head
Mathias Kalxdorf
contact affiliation	EMBL Heidelberg
contact email	mathiaskalxdorf@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD016249

PRIDE project URI

• PRIDE
  1. PXD016249
     1. Label: PRIDE project
     2. Name: Tracking drug action on the cell surface proteome