PXD016245

PXD016245 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis reveals core monocyte-derived cell differentiation proteome and highlights cellular identity-specific proteins
Description	Monocytes can give rise to multiple highly specialized cell types to perform a wide array of functions, ranging from pathogen phagocytosis to bone resorption. This differentiation is induced by the binding of cytokines to dedicated receptors on the surface of monocytes, which results in the initiation of genetic programs that enable cells to perform their specialized functions. Given their common background, it is not surprising that monocyte-derived cells share abilities and cellular markers, yet their specialized functions require a dedicated set of proteins. In order to dissect the monocyte differentiation process and to define cell type-specific marker proteins, we differentiated circulating monocytes into dendritic cells, M1 and M2 macrophages, and osteoclasts, and assessed their proteomes by quantitative mass spectrometry throughout the differentiation process. Statistical analysis indicated that monocyte differentiation is a linear process characterized by a common core of proteins that is similarly affected among the distinct differentiation paths. Throughout the specialization process a cluster of RNA-binding and processing proteins was downregulated whereas proteins associated to metabolic processes were increased. Analysis of the specialized cells after 10 days of differentiation uncovered existing and putative novel dendritic cell markers. Combined, we here present a comprehensive proteomic analysis of monocyte differentiation uncovering shared and distinct proteomic features of differentiating monocytes and monocyte-derived cells.
HostingRepository	PRIDE
AnnounceDate	2020-05-12
AnnouncementXML	Submission_2020-05-11_23:35:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eelke Béguin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-12 02:46:19	ID requested
⏵ 1	2020-05-11 23:35:14	announced

Dataset with its publication pending

submitter keyword: monocyte-derived cells, differentiation, proteomics, dendritic cells, M1 macrophages, M2 macrophages, osteoclasts

Maartje van den Biggelaar
contact affiliation	Molecular and Cellular Hemostasis, Sanquin Research, Amsterdam
contact email	m.vandenbiggelaar@sanquin.nl
lab head
Eelke Béguin
contact affiliation	Sanquin Research
contact email	e.beguin@sanquin.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD016245

PRIDE project URI

• PRIDE
  1. PXD016245
     1. Label: PRIDE project
     2. Name: Proteomic analysis reveals core monocyte-derived cell differentiation proteome and highlights cellular identity-specific proteins