PXD016240

PXD016240 is an original dataset announced via ProteomeXchange.

Title	Investigation of isoAsp accumulation to antioxidative enzymes during thermal stress and their repair by PROTEIN L-ISOASPARTYL METHYLTRANSFERASE (PIMT).
Description	The formation of isoAsp residues from Asp and Asn residues in proteins and peptides is accelerated under stressful environments and has detrimental effects on protein structure and function. PROTEIN L-ISOASPARTYL METHYLTRANSFERASE (PIMT) is a protein repairing enzyme which revert such deleterious isoAsp residues to normal aspartyl residues as a protein repair process. In present work, we aim to study the isoaspartyl formation of antioxidative enzymes, superoxide dismutase (SOD) and catalase (CAT) under stressful environments and their repair by PIMT in Arabidopsis. The role of PIMT in repairing these enzymes for the plant survival under heat and oxidative stress were investigated through molecular, biochemical approaches in Arabidopsis. We demonstrated that isoAsp accumulation in proteins increase upon heat and oxidative stress, and PIMT activity is essential to restrict such isoAsp accumulation. We demonstrated that PIMT play an important role under heat and oxidative stress by restricting stress-induced deleterious isoAsp accumulation in proteins. Analyses of PIMT overexpression and RNAi lines suggest that PIMT maintains ROS homeostasis by protecting the functionality of antioxidative enzymes from isoAsp mediated damage under stressful environments. To confirm further whether Asn residues of antioxidative enzymes indeed susceptible to isoAsp formation, we used bacterially expressed and purified recombinant proteins for MS analyses. Purified proteins were subjected to thermal insult and then MS/MS analyses were carried out to identify isoAsp modification. Our MS analyses suggest that these antioxidative enzymes are indeed susceptible to isoAsp formation under thermal stress.
HostingRepository	PRIDE
AnnounceDate	2019-12-18
AnnouncementXML	Submission_2019-12-18_05:09:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manoj Majee
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Q TRAP

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-12 02:43:36	ID requested
⏵ 1	2019-12-18 05:10:00	announced

Ghosh S, Kamble NU, Verma P, Salvi P, Petla BP, Roy S, Rao V, Hazra A, Varshney V, Kaur H, Majee M, protein l-ISOASPARTYL METHYLTRANSFERASE repairs isoaspartyl damage to antioxidant enzymes and increases heat and oxidative stress tolerance. J Biol Chem, 295(3):783-799(2020) [pubmed]

submitter keyword: PIMT, Antioxidative enzyme, Arabidopsis, isoAsp, Protein repair.

Dr. Manoj Majee
contact affiliation	National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067
contact email	manojmajee@nipgr.ac.in
lab head
Manoj Majee
contact affiliation	National Institute of Plant Genome Research, New Delhi
contact email	manojmajee@nipgr.ac.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016240
     1. Label: PRIDE project
     2. Name: Investigation of isoAsp accumulation to antioxidative enzymes during thermal stress and their repair by PROTEIN L-ISOASPARTYL METHYLTRANSFERASE (PIMT).