PXD016231

PXD016231 is an original dataset announced via ProteomeXchange.

Title	Proteomics of saliva, plasma, and salivary gland tissue in Sjögren’s syndrome and non-Sjögren patients identify novel biomarker candidates
Description	Objective: The aim of this study was to characterize and compare the proteome in whole saliva, plasma, and salivary gland tissue in patients with primary Sjögren’s syndrome (pSS) and patients having symptoms of pSS, but not fulfilling the classification criteria, and to search for diagnostic biomarker candidates for pSS. Methods: Liquid chromatography tandem mass spectrometry was conducted on whole saliva, plasma, and labial salivary gland tissue samples from 24 patients with pSS and 16 non-Sjögren control subjects (non-pSS). Gene Ontology (GO)-terms and Kyoto Encyclopedia of Genes and Genomes (KEGG)-pathways were applied for functional annotation. Results: 1,013 proteins were identified in whole saliva, 219 in plasma, and 3,166 in salivary gland tissue. In saliva, 40 proteins differed significantly between the two groups. In pSS, proteins involved in immunoinflammatory processes were upregulated, whereas proteins related to salivary secretion were downregulated. The combination of neutrophil elastase, calreticulin, and tripartite motif-containing protein 29 yielded a receiver-operating characteristic (ROC) value of 0.97 (CI 0.93-1.00). Protein expression in plasma and salivary gland tissue did not differ between the patient groups. Conclusion: The salivary proteome of patients with pSS differed from that of non-pSS patients, indicating that saliva proteomics represents a promising non-invasive diagnostic tool for pSS.
HostingRepository	PRIDE
AnnounceDate	2020-07-09
AnnouncementXML	Submission_2020-09-28_00:25:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maria Sembler-Møller
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-11 08:12:55	ID requested
1	2020-07-09 04:38:43	announced
⏵ 2	2020-09-28 00:25:11	announced	2020-09-28: Updated publication reference for PubMed record(s): 32540407.

Sembler-M, ø, ller ML, Belstr, ø, m D, Locht H, Pedersen AML, gren patients identify novel biomarker candidates. J Proteomics, 225():103877(2020) [pubmed]

submitter keyword: Sjögren’s syndrome, Saliva, Plasma, Minor Salivary Glands, Proteomics, Biomarker

Maria Lynn Sembler-Møller
contact affiliation	Section for Oral Medicine and Oral Pathology, Department of Odontology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
contact email	mlse@sund.ku.dk
lab head
Maria Sembler-Møller
contact affiliation	University of Copenhagen
contact email	mlse@sund.ku.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016231
     1. Label: PRIDE project
     2. Name: Proteomics of saliva, plasma, and salivary gland tissue in Sjögren’s syndrome and non-Sjögren patients identify novel biomarker candidates