PXD016230

PXD016230 is an original dataset announced via ProteomeXchange.

Title	Synaptic transmission induces site specific changes in sialylation on N-linked glycoproteins in nerve terminals
Description	Synaptic transmission is an ultra-fast process that aims at transducing a signal from one neuron to another neuron or cell by the release of neurotransmitters. This process requires rapid response which can be transmitted through fast molecular switches such as post-translational modifications of proteins. We aimed at investigating the potential importance in dynamic modulation of sialic acids on N-linked glycoproteins that are essential for the synaptic transmission process. For this, we characterize site-specific alteration in sialylated N-linked glycosylation in the active zone of rat nerve terminals (synaptosomes) after depolarization by using quantitative sialiomics.
HostingRepository	PRIDE
AnnounceDate	2020-06-11
AnnouncementXML	Submission_2020-06-11_15:18:39.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Inga Boll
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-11 08:11:50	ID requested
⏵ 1	2020-06-11 15:18:42	announced

Boll I, Jensen P, Schw, ä, mmle V, Larsen MR, linked Glycoproteins in Rat Nerve Terminals. Mol Cell Proteomics, 19(9):1418-1435(2020) [pubmed]

submitter keyword: Synaptosomes, Rat, LC-MS/MS, Sialic acid, N-glycosylation, synaptic transmission

Martin R. Larsen
contact affiliation	Department of Biochemistry and Molecular Biology, University of Southern Denmark, Denmark
contact email	mrl@bmb.sdu.dk
lab head
Inga Boll
contact affiliation	University of Southern Denmark
contact email	ingaboll@bmb.sdu.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016230
     1. Label: PRIDE project
     2. Name: Synaptic transmission induces site specific changes in sialylation on N-linked glycoproteins in nerve terminals