PXD016221

PXD016221 is an original dataset announced via ProteomeXchange.

Title	2019 Brecht et al. RAG1 vAbl BioID LC-MS/MS
Description	To identify mechanisms that regulate V(D)J recombination, we used proximity-dependent biotin identification to analyze the interactomes of full length and truncated forms of RAG1 in pre-B cells. This revealed an association of RAG1 with numerous nucleolar proteins in a manner dependent on amino acids 216-383 and allowed identification of a motif required for nucleolar localization. Experiments in transformed pre-B cell lines and cultured primary pre-B cells reveal a strong correlation between disruption of nucleoli, reduced association of RAG1 with a nucleolar marker, and increases in V(D)J recombination activity. Mutation of the RAG1 nucleolar localization motif boosts recombination while removal of the first 215 amino acids of RAG1, which are required for efficient egress from nucleoli, reduces recombination activity. Our findings indicate that nucleolar sequestration of RAG1 is a negative regulatory mechanism in V(D)J recombination and identify regions of the RAG1 N-terminal region that control nucleolar association and egress.
HostingRepository	PRIDE
AnnounceDate	2020-02-17
AnnouncementXML	Submission_2020-02-17_01:54:32.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD016221
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ryan Brecht
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-11 02:36:44	ID requested
⏵ 1	2020-02-17 01:54:34	announced

Brecht RM, Liu CC, Beilinson HA, Khitun A, Slavoff SA, Schatz DG, Nucleolar localization of RAG1 modulates V(D)J recombination activity. Proc Natl Acad Sci U S A, 117(8):4300-4309(2020) [pubmed]

submitter keyword: RAG1, V(D)J Recombination, BioID, vAbl Cell, Pre B Cell

David Schatz
contact affiliation	Chair of Immunobiology, Yale University
contact email	david.schatz@yale.edu
lab head
Ryan Brecht
contact affiliation	Yale University
contact email	Ryan.Brecht@yale.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/02/PXD016221

PRIDE project URI

• PRIDE
  1. PXD016221
     1. Label: PRIDE project
     2. Name: 2019 Brecht et al. RAG1 vAbl BioID LC-MS/MS