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PXD016204

PXD016204 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleOuter membrane proteome of Leptospira; revealing the bottom of iceberg
DescriptionLeptospira, the causative agent of leptospirosis is known to have several proteases with potential to degrade extracellular matrix. However, a multipronged approach to identify, classify, characterize and elucidate their role has not been attempted. In this study, we carried out in-depth proteomic analysis of Triton X-114 fractions of Leptospirainterrogansusing high-resolution LC-MS/MS. Our analysis resulted in the identification of We have identified 2957 proteins that account 80.6% proteins of Leptospira and their subcellular distribution. The analysis showed differential enrichment of proteins in aqueous, detergent and pellet fractions with respect to the subcellular localization predicted by on CELLO v.2.5. It is found that highest number of OMP and pathogenic protein species were found in aqueous and pellet representing the cytoplasm and inner membranewhile the detergent fraction representing the OM contain highest amount of OMPs and pathogenic proteins even though the number of protein species were less. Thus the higher number of OMP and pathogenic proteins in the inner compartments of the cell carry the possibility of their deployment on the OM in abundance under pathogenic conditions.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:11:41.295.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMadathiparambil G. Madanan
SpeciesList scientific name: Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130; NCBI TaxID: 267671;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-11-08 01:33:22ID requested
12020-09-01 04:53:38announced
22024-10-22 05:11:42announced2024-10-22: Updated project metadata.
Publication List
Thoduvayil S, Dhandapani G, Brahma R, Devasahayam Arokia Balaya R, Mangalaparthi KK, Patel K, Kumar M, Tennyson J, Satheeshkumar PK, Kulkarni MJ, Pinto SM, Prasad TSK, Madanan MG, Triton X-114 Fractionated Subcellular Proteome of Leptospira interrogans Shows Selective Enrichment of Pathogenic and Outer Membrane Proteins in the Detergent Fraction. Proteomics, 20(19-20):e2000170(2020) [pubmed]
10.1002/pmic.202000170;
Keyword List
submitter keyword: Pathogenic high resolution proteomics, protease, pathogenesis, Outer Membrane,Leptospira
Contact List
Dr M. G. Madanan
contact affiliationScientist E Regional Medical Research Centre, Indian Council of Medical Research Port Blair, INDIA
contact emailmadananmg@icmr.org.in
lab head
Madathiparambil G. Madanan
contact affiliationIndian Council of Medical Research, Regional Medical Research Centre
contact emailmadananmg@icmr.org.in
dataset submitter
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Dataset FTP location
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