PXD016204 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Outer membrane proteome of Leptospira; revealing the bottom of iceberg |
Description | Leptospira, the causative agent of leptospirosis is known to have several proteases with potential to degrade extracellular matrix. However, a multipronged approach to identify, classify, characterize and elucidate their role has not been attempted. In this study, we carried out in-depth proteomic analysis of Triton X-114 fractions of Leptospirainterrogansusing high-resolution LC-MS/MS. Our analysis resulted in the identification of We have identified 2957 proteins that account 80.6% proteins of Leptospira and their subcellular distribution. The analysis showed differential enrichment of proteins in aqueous, detergent and pellet fractions with respect to the subcellular localization predicted by on CELLO v.2.5. It is found that highest number of OMP and pathogenic protein species were found in aqueous and pellet representing the cytoplasm and inner membranewhile the detergent fraction representing the OM contain highest amount of OMPs and pathogenic proteins even though the number of protein species were less. Thus the higher number of OMP and pathogenic proteins in the inner compartments of the cell carry the possibility of their deployment on the OM in abundance under pathogenic conditions. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:11:41.295.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Madathiparambil G. Madanan |
SpeciesList | scientific name: Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130; NCBI TaxID: 267671; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-11-08 01:33:22 | ID requested | |
1 | 2020-09-01 04:53:38 | announced | |
⏵ 2 | 2024-10-22 05:11:42 | announced | 2024-10-22: Updated project metadata. |
Publication List
Thoduvayil S, Dhandapani G, Brahma R, Devasahayam Arokia Balaya R, Mangalaparthi KK, Patel K, Kumar M, Tennyson J, Satheeshkumar PK, Kulkarni MJ, Pinto SM, Prasad TSK, Madanan MG, Triton X-114 Fractionated Subcellular Proteome of Leptospira interrogans Shows Selective Enrichment of Pathogenic and Outer Membrane Proteins in the Detergent Fraction. Proteomics, 20(19-20):e2000170(2020) [pubmed] |
10.1002/pmic.202000170; |
Keyword List
submitter keyword: Pathogenic high resolution proteomics, protease, pathogenesis, Outer Membrane,Leptospira |
Contact List
Dr M. G. Madanan |
contact affiliation | Scientist E Regional Medical Research Centre, Indian Council of Medical Research Port Blair, INDIA |
contact email | madananmg@icmr.org.in |
lab head | |
Madathiparambil G. Madanan |
contact affiliation | Indian Council of Medical Research, Regional Medical Research Centre |
contact email | madananmg@icmr.org.in |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD016204 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016204
- Label: PRIDE project
- Name: Outer membrane proteome of Leptospira; revealing the bottom of iceberg