PXD016204

PXD016204 is an original dataset announced via ProteomeXchange.

Title	Outer membrane proteome of Leptospira; revealing the bottom of iceberg
Description	Leptospira, the causative agent of leptospirosis is known to have several proteases with potential to degrade extracellular matrix. However, a multipronged approach to identify, classify, characterize and elucidate their role has not been attempted. In this study, we carried out in-depth proteomic analysis of Triton X-114 fractions of Leptospirainterrogansusing high-resolution LC-MS/MS. Our analysis resulted in the identification of We have identified 2957 proteins that account 80.6% proteins of Leptospira and their subcellular distribution. The analysis showed differential enrichment of proteins in aqueous, detergent and pellet fractions with respect to the subcellular localization predicted by on CELLO v.2.5. It is found that highest number of OMP and pathogenic protein species were found in aqueous and pellet representing the cytoplasm and inner membranewhile the detergent fraction representing the OM contain highest amount of OMPs and pathogenic proteins even though the number of protein species were less. Thus the higher number of OMP and pathogenic proteins in the inner compartments of the cell carry the possibility of their deployment on the OM in abundance under pathogenic conditions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:11:41.295.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Madathiparambil G. Madanan
SpeciesList	scientific name: Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130; NCBI TaxID: 267671;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-08 01:33:22	ID requested
1	2020-09-01 04:53:38	announced
⏵ 2	2024-10-22 05:11:42	announced	2024-10-22: Updated project metadata.

Thoduvayil S, Dhandapani G, Brahma R, Devasahayam Arokia Balaya R, Mangalaparthi KK, Patel K, Kumar M, Tennyson J, Satheeshkumar PK, Kulkarni MJ, Pinto SM, Prasad TSK, Madanan MG, Triton X-114 Fractionated Subcellular Proteome of Leptospira interrogans Shows Selective Enrichment of Pathogenic and Outer Membrane Proteins in the Detergent Fraction. Proteomics, 20(19-20):e2000170(2020) [pubmed]

10.1002/pmic.202000170;

submitter keyword: Pathogenic high resolution proteomics, protease, pathogenesis, Outer Membrane,Leptospira

Dr M. G. Madanan
contact affiliation	Scientist E Regional Medical Research Centre, Indian Council of Medical Research Port Blair, INDIA
contact email	madananmg@icmr.org.in
lab head
Madathiparambil G. Madanan
contact affiliation	Indian Council of Medical Research, Regional Medical Research Centre
contact email	madananmg@icmr.org.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD016204

PRIDE project URI

• PRIDE
  1. PXD016204
     1. Label: PRIDE project
     2. Name: Outer membrane proteome of Leptospira; revealing the bottom of iceberg