PXD016182
PXD016182 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | rat neutrophils phosphoproteome analysis |
| Description | Intestinal ischemia reperfusion injury (iIRI) is a severe clinical condition presenting high morbidity and mortality worldwide. Some of the systemic consequences of iIRI can be prevented by applying ischemic preconditioning (iIPC), a series of short ischemia/reperfusion events preceding the major ischemia. Although neutrophils are key players in the physiopathology of ischemic injuries, neither the dysregulation presented by these cells in iIRI nor the protective effect of iIPC have their regulation mechanisms fully understood. Protein phosphorylation, as well as the regulation of the respective phosphatases and kinases are responsible for regulating a large number of cellular functions in the inflammatory response. Therefore, in the present study we analyzed the phosphoproteome of neutrophils from rats submitted to mesenteric ischemia and reperfusion, either submitted or not to IPC, compared to quiescent controls and sham laparotomy. Proteomic analysis was performed by multi-step enrichment of phosphopeptides, isobaric labeling and LC-MS/MS analysis. Bioinformatics was used to determine phosphosite and phosphopeptide abundance and clustering, as well as kinases and phosphatases sites and domains. We found that most of the phosphorylation-regulated proteins are involved in apoptosis and migration, and most of the regulatory kinases belong to CAMK and CMGC families. Some relevant regulatory proteins discussed are Vamp8, PKC delta, NDR1/Stk38 and Inpp5c/Shipp |
| HostingRepository | MassIVE |
| AnnounceDate | 2022-08-07 |
| AnnouncementXML | Submission_2022-08-07_09:02:49.789.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Laboratorio de Bioquimica e Quimica de Proteinas |
| SpeciesList | scientific name: Rattus norvegicus; common name: Norway rat; NCBI TaxID: 10116; |
| ModificationList | Phospho; Carbamidomethyl; Oxidation |
| Instrument | LTQ Orbitrap Velos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2019-11-06 12:59:28 | ID requested | |
| ⏵ 1 | 2022-08-07 09:02:50 | announced |
Publication List
| Tahir M, Arshid S, Fontes B, S Castro M, Sidoli S, Schw, รค, mmle V, Luz IS, Roepstorff P, Fontes W, Phosphoproteomic Analysis of Rat Neutrophils Shows the Effect of Intestinal Ischemia/Reperfusion and Preconditioning on Kinases and Phosphatases. Int J Mol Sci, 21(16):(2020) [pubmed] |
Keyword List
| submitter keyword: inflammatory response, neutrophil, phosphatases, kinases |
Contact List
| Wagner Fontes | |
|---|---|
| contact affiliation | UnB |
| contact email | wagnerfontes2@gmail.com |
| lab head | |
| Laboratorio de Bioquimica e Quimica de Proteinas | |
| contact affiliation | UnB |
| contact email | LBQP.Massive@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084545/ |
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