PXD016174

PXD016174 is an original dataset announced via ProteomeXchange.

Title	Sgt1 interactome analysis under proteostatic stress and steady state conditions
Description	Buildup of misfolded proteins are prevented by chaperone-assisted refolding and/or proteasome dependent degradation. The coordination of these two protein quality control (PQC) systems is not fully understood. We identified the essential Hsp90 co-chaperone Sgt1 as a new member of a general PQC linking folding and degradation. Upon proteostatic stress, e.g. heat shock, Sgt1 accumulates in an Hsp90- and proteasome dependent manner at an until now unknown spatial PQC compartment in both yeast and human cells. In order to find further components of this new PQC compartment we performed pull down experiments and a protein interaction analysis of cells expressing either GFP tagged Sgt1 (or GFP alone, as a negative control) that were either grown at 30°C or under heat shock conditions (42°C, 30 minutes).
HostingRepository	PRIDE
AnnounceDate	2021-07-02
AnnouncementXML	Submission_2021-07-01_23:44:09.706.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frank Stein
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-06 04:00:33	ID requested
⏵ 1	2021-07-01 23:44:10	announced

Eisele F, Eisele-B, ü, rger AM, Hao X, Berglund LL, H, ö, ö, g JL, Liu B, Nystr, ö, m T, An Hsp90 co-chaperone links protein folding and degradation and is part of a conserved protein quality control. Cell Rep, 35(13):109328(2021) [pubmed]

curator keyword: Biological

submitter keyword: Sgt1, heat shock, HAPI, Protein quality control, Saccharomyces cerevisiae

Thomas Nystroem
contact affiliation	Department of Immunology and Microbiology Institute of Biomedicine Sahlgrenska Academy University of Gothenburg
contact email	thomas.nystrom@gu.se
lab head
Frank Stein
contact affiliation	EMBL
contact email	frank.stein@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD016174

PRIDE project URI

• PRIDE
  1. PXD016174
     1. Label: PRIDE project
     2. Name: Sgt1 interactome analysis under proteostatic stress and steady state conditions