PXD016167

PXD016167 is an original dataset announced via ProteomeXchange.

Title	LC-MS/MS of baicalein-regulated human lung fibroblast
Description	Here, we showed that baicalein suppressed transforming growth factor β1 (TGF β1)-stimulated the production of type I collagen in lung fibroblast MRC-5 cells. By applying SILAC-based proteomic technology, 158 proteins were identified as baicalein-modulated proteins in TGF β1-stimulated the accumulation of type I collagen in MRC-5 cells. Our proteomic and biochemical analysis demonstrated that baicalein could decrease the expression levels of connective tissue growth factor (CTGF) in TGF β1-stimulated MRC-5 cells. In addition, CTGF overexpression elevated the levels of type I collagen in baicalein-treated fibroblasts. Moreover, our results demonstrated that baicalein-downregulated CTGF expression might be related with the decrease of Smad2 phosphorylation, but not SP1.
HostingRepository	PRIDE
AnnounceDate	2020-09-25
AnnouncementXML	Submission_2020-09-25_03:09:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaogang Jiang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-06 02:06:02	ID requested
⏵ 1	2020-09-25 03:09:33	announced

Sun X, Cui X, Chen X, Jiang X, 1-induced type I collagen production in lung fibroblasts via downregulation of connective tissue growth factor. Biomed Pharmacother, 131():110744(2020) [pubmed]

submitter keyword: Human

lung fibroblast

baicalein

LC-MS/MS

Xiaogang Jiang
contact affiliation	Department of Pharmacology, Soochow University
contact email	jiangxiaogang@suda.edu.cn
lab head
Xiaogang Jiang
contact affiliation	Soochow University
contact email	jiangxiaogang@suda.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD016167

PRIDE project URI

• PRIDE
  1. PXD016167
     1. Label: PRIDE project
     2. Name: LC-MS/MS of baicalein-regulated human lung fibroblast