PXD016158 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantification of exercise-regulated ubiquitin-signalling |
Description | The maintenance of muscle function is extremely important for whole body health and exercise is essential to this process. The ubiquitin-proteasome system (UPS) is required for muscle adaptation following exercise but little is known about acute post-translational regulation and proteome remodelling during exercise. Here, we used quantitative proteomics to study ubiquitin-signalling dynamics in human skeletal muscle biopsies from healthy males before, during and after a single bout of high-intensity exercise. Exercise resulted in a marked depletion of protein ubiquitylation consistent with proteasome activation. This was also associated with acute post-translational modification of protein abundance via a network of proteases. |
HostingRepository | PRIDE |
AnnounceDate | 2020-03-17 |
AnnouncementXML | Submission_2020-03-17_03:57:44.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Benjamin Parker |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-11-05 08:15:40 | ID requested | |
⏵ 1 | 2020-03-17 03:57:45 | announced | |
Publication List
Parker BL, Kiens B, Wojtaszewski JFP, Richter EA, James DE, Quantification of exercise-regulated ubiquitin signaling in human skeletal muscle identifies protein modification cross talk via NEDDylation. FASEB J, 34(4):5906-5916(2020) [pubmed] |
Keyword List
submitter keyword: exercise, ubiquitin, proteomics |
Contact List
Benjamin Parker |
contact affiliation | The University of Melbourne and University of Sydney |
contact email | ben.parker@unimelb.edu.au |
lab head | |
Benjamin Parker |
contact affiliation | The University of Melbourne |
contact email | ben.parker@unimelb.edu.au |
dataset submitter | |
Full Dataset Link List
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016158
- Label: PRIDE project
- Name: Quantification of exercise-regulated ubiquitin-signalling