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PXD016158 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantification of exercise-regulated ubiquitin-signalling
DescriptionThe maintenance of muscle function is extremely important for whole body health and exercise is essential to this process. The ubiquitin-proteasome system (UPS) is required for muscle adaptation following exercise but little is known about acute post-translational regulation and proteome remodelling during exercise. Here, we used quantitative proteomics to study ubiquitin-signalling dynamics in human skeletal muscle biopsies from healthy males before, during and after a single bout of high-intensity exercise. Exercise resulted in a marked depletion of protein ubiquitylation consistent with proteasome activation. This was also associated with acute post-translational modification of protein abundance via a network of proteases.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBenjamin Parker
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-11-05 08:15:40ID requested
12020-03-17 03:57:45announced
Publication List
Parker BL, Kiens B, Wojtaszewski JFP, Richter EA, James DE, Quantification of exercise-regulated ubiquitin signaling in human skeletal muscle identifies protein modification cross talk via NEDDylation. FASEB J, 34(4):5906-5916(2020) [pubmed]
Keyword List
submitter keyword: exercise, ubiquitin, proteomics
Contact List
Benjamin Parker
contact affiliationThe University of Melbourne and University of Sydney
contact emailben.parker@unimelb.edu.au
lab head
Benjamin Parker
contact affiliationThe University of Melbourne
contact emailben.parker@unimelb.edu.au
dataset submitter
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