PXD016151

PXD016151 is an original dataset announced via ProteomeXchange.

Title	Identifying Sialylation Linkages at the Glycopeptide Level by Glycosyltransferase Labeling Assisted Mass Spectrometry
Description	Precise assignment of sialylation linkages at the glycopeptide level is of importance in bottom-up glycoproteomics, and is also an indispensable step to understand the function of glycoproteins in pathogen-host interactions and cancer progression. Even though some efforts have been dedicated to the discrimination of α2,3/α2,6-sialylated isomers, unambiguous identification of sialoglycopeptide isomers is still needed. Herein, an innovative strategy of glycosyltransferase labeling assisted mass spectrometry (GLAMS) was developed. After specific enzymatic labeling, oxonium ions from higher-energy C-trap dissociation (HCD) fragmentation of α2,3-sailoglycopeptides generate unique reporters to distinctly differentiate those of α2,6-sailoglycopeptide isomers.
HostingRepository	PRIDE
AnnounceDate	2020-04-21
AnnouncementXML	Submission_2020-04-21_01:34:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	He Zhu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-05 08:12:55	ID requested
⏵ 1	2020-04-21 01:34:20	announced

Zhu H, Wang S, Liu D, Ding L, Chen C, Liu Y, Wu Z, Bollag R, Liu K, Alexander WM, Yin J, Ma C, Li L, Wang PG, Identifying Sialylation Linkages at the Glycopeptide Level by Glycosyltransferase Labeling Assisted Mass Spectrometry (GLAMS). Anal Chem, 92(9):6297-6303(2020) [pubmed]

submitter keyword: human serum, glycopeptides, site-specific sialylation, glycosyltransferase labeling

Peng George Wang
contact affiliation	George State University
contact email	pwang11@gsu.edu
lab head
He Zhu
contact affiliation	Georgia State University
contact email	glycohezhu@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD016151

PRIDE project URI

• PRIDE
  1. PXD016151
     1. Label: PRIDE project
     2. Name: Identifying Sialylation Linkages at the Glycopeptide Level by Glycosyltransferase Labeling Assisted Mass Spectrometry