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PXD016149

PXD016149 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTFEB regulates protein synthesis to sustain autophagy during starvation
DescriptionIn response to starvation, cells undergo a metabolic shift to ensure their survival by shutting down protein synthesis and activating catabolic processes, including autophagy, to degrade proteins and recycle nutrients. These processes, however, do require protein synthesis. We asked how this fundamental conflict is resolved. Upon starvation, cells activate the Integrated Stress Response (ISR) and inhibit mammalian target of rapamycin complex 1 (mTORC1). The ISR inhibits protein translation through the phosphorylation of the alpha subunit of eukaryotic translation initiation factor 2 (eIF2), whereas mTORC1 inhibition induces activation of the transcription factors TFEB/TFE3. We discovered that PPP1R15A (aka GADD34), a member of the protein phosphatase 1 complex (PP1) is a direct and early target of TFEB. GADD34 recruits PP1 to dephosphorylate eIF2 and thus fine-tunes protein synthesis to enable translation of the transcriptional program induced by starvation leading to a sustained autophagic flux.
HostingRepositoryPRIDE
AnnounceDate2020-09-30
AnnouncementXMLSubmission_2020-09-29_23:37:17.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAndrea Armirotti
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-11-05 08:12:21ID requested
12020-09-29 23:37:18announced
Publication List
Gambardella G, Staiano L, Moretti MN, De Cegli R, Fagnocchi L, Di Tullio G, Polletti S, Braccia C, Armirotti A, Zippo A, Ballabio A, De Matteis MA, di Bernardo D, GADD34 is a modulator of autophagy during starvation. Sci Adv, 6(39):(2020) [pubmed]
Keyword List
submitter keyword: Human HEK293
Contact List
Andrea Armirotti
contact affiliationAnalytical Chemistry Lab, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy
contact emailandrea.armirotti@iit.it
lab head
Andrea Armirotti
contact affiliationAnalytical Chemistry Lab, Fondazione Istituto Italiano di Tecnologia
contact emailandrea.armirotti@iit.it
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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