PXD016149 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | TFEB regulates protein synthesis to sustain autophagy during starvation |
Description | In response to starvation, cells undergo a metabolic shift to ensure their survival by shutting down protein synthesis and activating catabolic processes, including autophagy, to degrade proteins and recycle nutrients. These processes, however, do require protein synthesis. We asked how this fundamental conflict is resolved. Upon starvation, cells activate the Integrated Stress Response (ISR) and inhibit mammalian target of rapamycin complex 1 (mTORC1). The ISR inhibits protein translation through the phosphorylation of the alpha subunit of eukaryotic translation initiation factor 2 (eIF2), whereas mTORC1 inhibition induces activation of the transcription factors TFEB/TFE3. We discovered that PPP1R15A (aka GADD34), a member of the protein phosphatase 1 complex (PP1) is a direct and early target of TFEB. GADD34 recruits PP1 to dephosphorylate eIF2 and thus fine-tunes protein synthesis to enable translation of the transcriptional program induced by starvation leading to a sustained autophagic flux. |
HostingRepository | PRIDE |
AnnounceDate | 2020-09-30 |
AnnouncementXML | Submission_2020-09-29_23:37:17.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Andrea Armirotti |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-11-05 08:12:21 | ID requested | |
⏵ 1 | 2020-09-29 23:37:18 | announced | |
Publication List
Gambardella G, Staiano L, Moretti MN, De Cegli R, Fagnocchi L, Di Tullio G, Polletti S, Braccia C, Armirotti A, Zippo A, Ballabio A, De Matteis MA, di Bernardo D, GADD34 is a modulator of autophagy during starvation. Sci Adv, 6(39):(2020) [pubmed] |
Keyword List
submitter keyword: Human HEK293 |
Contact List
Andrea Armirotti |
contact affiliation | Analytical Chemistry Lab, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy |
contact email | andrea.armirotti@iit.it |
lab head | |
Andrea Armirotti |
contact affiliation | Analytical Chemistry Lab, Fondazione Istituto Italiano di Tecnologia |
contact email | andrea.armirotti@iit.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016149
- Label: PRIDE project
- Name: TFEB regulates protein synthesis to sustain autophagy during starvation