PXD016147

PXD016147 is an original dataset announced via ProteomeXchange.

Title	BAP and ADCK4 interactor proteomic data LC-MS/MS
Description	Although identification of single gene mutations associated with SRNS have yielded insights into pathogenic mechanisms and localized its pathogenesis to glomerular podocytes, the disease mechanisms of SRNS remain obscure. ADCK4 mutations usually manifest as steroid-resistant nephrotic syndrome, and cause coenzyme Q10 (CoQ10) deficiency. We performed proteomic analysis to understand the function of ADCK4 via the identification of its interactome. We generated HEK293 cells that stably overexpressed C-terminal FLAG-tagged bacterial alkaline phosphatase (BAP; BAP-3xFLAG) and ADCK4 (ADCK4-3xFLAG). We confirmed that ADCK4-3xFLAG mostly localized to the mitochondria. Following affinity purification using anti-FLAG beads, protein eluates were analyzed using a liquid chromatograph coupled to a high-resolution mass spectrometer (LC-MS/MS). In total, 612 proteins were identified as interactors of ADCK4. Among them, the cytoplasmic proteins, including myosin (MYH10, MYH11, MYO1B, and MYO1C), filamin (FLNC), and kinase proteins (STK24, STK25, STK38 and ROCK1) were detected. In addition, the mitochondrial proteins, including ATP synthase subunit (ATP5L), cytochrome c oxidase subunit (COX6A1 and UQCRQ), and COQ5, were also identified as interactors.
HostingRepository	PRIDE
AnnounceDate	2021-09-08
AnnouncementXML	Submission_2021-09-08_13:54:46.338.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD016147
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	SEYOUNG YU
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Carbamidomethyl
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-05 06:44:43	ID requested
⏵ 1	2021-09-08 13:54:50	announced

Widmeier E, Yu S, Nag A, Chung YW, Nakayama M, Fern, á, ndez-Del-R, í, o L, Hugo H, Schapiro D, Buerger F, Choi WI, Helmst, ä, dter M, Kim JW, Ryu JH, Lee MG, Clarke CF, Hildebrandt F, Gee HY, ADCK4 Deficiency Destabilizes the Coenzyme Q Complex, Which Is Rescued by 2,4-Dihydroxybenzoic Acid Treatment. J Am Soc Nephrol, 31(6):1191-1211(2020) [pubmed]

submitter keyword: BAP, ADCK4, LC-MS/MS, FLAG, IP, human

Gee Heon Yung
contact affiliation	Department of Pharmacology, Brain Korea 21 PLUS Project for Medical Sciences, Yonsei University College of Medicine, Seoul 03722, Korea
contact email	hygee@yuhs.ac
lab head
SEYOUNG YU
contact affiliation	Department of Pharmacology, Brain Korea 21 PLUS Project for Medical Sciences, Yonsei University College of Medicine, Seoul 03722, Korea
contact email	goldsy420@yuhs.ac
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016147
     1. Label: PRIDE project
     2. Name: BAP and ADCK4 interactor proteomic data LC-MS/MS