PXD016146

PXD016146 is an original dataset announced via ProteomeXchange.

Title	MALDI rat liver anticancer drug spiked-in dataset (imzML)
Description	For the MALDI-MSI experiment, we selected 12 different drugs. The drugs were purchased from the LC Laboratories (Woburn, MA; CAS numbers: dabrafenib: 1195765-45-7, dasatinib: 302962-49-8, erlotinib: 183321-74-6, gefitinib: 184475-35-2, imatinib: 152459-95-5, lapatinib: 388082-78-8, pazopanib: 444731-52-6, sorafenib: 284461-73-0, sunitinib: 557795-19-4, trametinib: 871700-17-3, vatalanib: 212141-54-3) and from SelleckChem (Munich, Germany; CAS numbers: ipratropium: 60205-81-4) with >99% purity and were dissolved in methanol (MeOH, (Chromasolv Plus for HPLC) (Sigma-Aldrich, Steinheim, Germany) at 10 mg/mL concentration. These stock solutions were further diluted with 50% MeOH and five mixtures were generated, each containing four different drug compounds. The spreadsheet in Supporting Information summarizes the composition of the five drug mixtures. A 5 mg/mL solution of ??-cyano-4-hydroxycinnamic acid (CHCA, Sigma-Aldrich) dissolved in 50% MeOH containing 0.1% trifluoroacetic acid (TFA, Sigma-Aldrich, Steinheim, Germany) was used as matrix solution.
HostingRepository	PRIDE
AnnounceDate	2019-11-27
AnnouncementXML	Submission_2019-11-27_06:00:36.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jonatan Eriksson
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-05 05:55:35	ID requested
1	2019-11-06 05:27:10	announced
⏵ 2	2019-11-27 06:00:37	announced	2019-11-27: Updated project metadata.

Eriksson JO, Rezeli M, Hefner M, Marko-Varga G, Horvatovich P, Clusterwise Peak Detection and Filtering Based on Spatial Distribution To Efficiently Mine Mass Spectrometry Imaging Data. Anal Chem, 91(18):11888-11896(2019) [pubmed]

submitter keyword: imzML ground truth spiked-in drugs

Peter Horvatovich
contact affiliation	University of Groningen, Department of Analytical Biochemistry, Groningen Research Institute of Pharmacy, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands Lund University, Department of Biomedical Engineering, Lund, Sweden
contact email	p.l.horvatovich@rug.nl
lab head
Jonatan Eriksson
contact affiliation	Lund University
contact email	jonatan.eriksson@bme.lth.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD016146

PRIDE project URI

• PRIDE
  1. PXD016146
     1. Label: PRIDE project
     2. Name: MALDI rat liver anticancer drug spiked-in dataset (imzML)