PXD016143

PXD016143 is an original dataset announced via ProteomeXchange.

Title	NETOME: The Molecular Characterization of Neutrophil Extracellular Traps (NETs)
Description	Neutrophils are necessary in mamalian’s life and are the most abundant type of white blood cells in humans with biological roles relevant to inflammation and the entire host response. The release of neutrophil extracellular DNA in innate immune cells provides specific response to bacteria and fungi. Neutrophil Extracellular Traps (NETs) act as antimicrobial agents and activators of immune response through release of the nuclear content into the extracellular space. Although great strides have been made in dissecting cellular and molecular pathways that control NET formation, the exact molecular composition of released NETs has not been elucidated. Here, we open the field of NETOMIC studies through isolation of NETs in combination with shotgun genomics and proteomics. This study reveals the molecular composition of NETs and specific expression regions of NETs induced in a sterile inflammation system. The existence of an in vitro NET isolation model allowed for an unprecedented amount of replicability. Additional studies are needed to verify the specificity of these sequences in the context of human health and disease upon diverse neutrophil microbial challenges.
HostingRepository	PRIDE
AnnounceDate	2022-10-25
AnnouncementXML	Submission_2022-10-25_09:18:16.466.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	YanbaoYu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-05 01:30:36	ID requested
⏵ 1	2022-10-25 09:18:16	announced

Dataset with its publication pending

submitter keyword: Genomics,Nets, Neutrophils, Extracellular DNA, Proteomics, Netome

MarceloFreire
contact affiliation	J. Craig Venter Institute 4120 Capricorn Lane La Jolla, CA 92037
contact email	mfreire@jcvi.org
lab head
YanbaoYu
contact affiliation	University of Delaware
contact email	yayu@jcvi.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD016143

PRIDE project URI

• PRIDE
  1. PXD016143
     1. Label: PRIDE project
     2. Name: NETOME: The Molecular Characterization of Neutrophil Extracellular Traps (NETs)