PXD016139
PXD016139 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | ATF4 Promotes Skeletal Muscle Atrophy by Forming a Heterodimer with C/EBPbeta |
| Description | Skeletal muscle atrophy is a highly prevalent and debilitating condition that remains poorly understood at the molecular level. Previous work found that skeletal muscle atrophy involves activating transcription factor 4 (ATF4), a protein in the basic leucine zipper (bZIP) transcription factor family. However, the direct biochemical mechanism by which ATF4 promotes muscle atrophy was unknown. Because bZIP proteins such as ATF4 must dimerize to bind and activate genes, and because ATF4 is unable to form highly stable homodimers, we hypothesized that ATF4 may promote muscle atrophy by heterodimerizing with another bZIP family member. To test this hypothesis, we biochemically isolated skeletal muscle proteins that associate with the dimerization- and DNA-binding domain of ATF4 (the bZIP domain) in mouse skeletal muscle fibers in vivo. Interestingly, we found that ATF4 makes up one half of at least 5 distinct heterodimeric bZIP transcription factors in skeletal muscle fibers. This three-way interaction between ATF4, C/EBPbeta and the ATF4-C/EBP composite site activates the Gadd45a gene, which encodes a known mediator of muscle atrophy (Gadd45a). Together, these results identify a direct biochemical mechanism by which ATF4 induces skeletal muscle atrophy and provide new insight into the way that skeletal muscle atrophy occurs at the molecular level. |
| HostingRepository | MassIVE |
| AnnounceDate | 2020-01-26 |
| AnnouncementXML | Submission_2020-01-26_10:51:46.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Birgit Schilling |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | TripleTOF 6600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2019-11-04 17:33:24 | ID requested | |
| ⏵ 1 | 2020-01-26 10:51:47 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: sarcopenia, data-independent acquisition, Skyline, Interacting proteins, bzip domaine |
Contact List
| Birgit Schilling | |
|---|---|
| contact affiliation | Buck Institute for Research on Aging |
| contact email | bschilling@buckinstitute.org |
| lab head | |
| Birgit Schilling | |
| contact affiliation | Buck Institute |
| contact email | bschilling@buckinstitute.org |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084538/ |
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