PXD016138

PXD016138 is an original dataset announced via ProteomeXchange.

Title	Measurement of potentially diagnostic organ-specific acute-phase blood protein level changes in early Lyme disease (Zhou et al. 2019)
Description	To identify blood proteins with altered abundances in patients with early stage Lyme disease as compared to healthy controls with the intent of identifying potential Lyme diagnostic markers, selected proteins from two categories were monitored by SRM: 1) key innate immunity regulators including acute phase proteins, reflecting the fact that LD is an acute inflammatory disease; and 2) organ-specific blood proteins predominantly expressed in organs and tissues known to be affected by B. burgdorferi, i.e., liver, heart, brain and skin. In all, 174 peptides (representing 124 human proteins) were measured in individual serum samples from 2 independent Lyme disease patient cohorts. Each sample set contains 30-40 confirmed Lyme disease patients (each with 4 time points) and 20 healthy controls (each with 2 time points) from baseline to 4-6 years years post-treatment. 90 proteotypic peptides from 73 targeted proteins were successfully detected and quantified. Six proteins (APOA4, C9, CRP, CST6, PGLYRP2 and S100A9) were confirmed to show significantly altered serum levels in patients at time of presentation. A 9-protein panel was also identified and verified.
HostingRepository	PeptideAtlas
AnnounceDate	2019-11-04
AnnouncementXML	Submission_2019-11-04_08:58:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yong Zhou
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine
Instrument	6490 Triple Quadrupole LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-04 08:48:02	ID requested
⏵ 1	2019-11-04 08:58:03	announced

Zhou Y, Qin S, Sun M, Tang L, Yan X, Kim TK, Caballero J, Glusman G, Brunkow ME, Soloski MJ, Rebman AW, Scavarda C, Cooper D, Omenn GS, Moritz RL, Wormser GP, Price ND, Aucott JN, Hood L, Measurement of Organ-Specific and Acute-Phase Blood Protein Levels in Early Lyme Disease. J Proteome Res, 19(1):346-359(2020) [pubmed]

curator keyword: selected reaction monitoring, SRM, targeted

Yong Zhou
contact affiliation	Institute for Systems Biology
contact email	yzhou@systemsbiology.org
dataset submitter
Leroy Hood
contact affiliation	Institute for Systems Biology
contact email	lhood@systemsbiology.org
lab head

PeptideAtlas dataset URI

PASSEL experiment URI

PASSEL transition group browser URI