PXD016135

PXD016135 is an original dataset announced via ProteomeXchange.

Title	Proteomics of regenerated tissue in response to a titanium implant with a bioactive surface in a rat tibial defect model
Description	Background: Due to their excellent mechanical and biocompatibility properties, titanium-based implants are successfully used as biomedical devices. However, when new bone formation fails for different reasons, impaired fracture healing will become a clinical problem and will affect the patient's quality of life. This study proposes to design a new bioactive surface of titanium implants with a synergetic PEG biopolymer-based composition for gradual delivery of growth factors (FGF2, VEGF, and BMP4) during bone healing. Methods: The optimal architecture of non-cytotoxic polymeric coatings deposited by dip coating under controlled parameters was assessed both in cultured cells and on rat in vivo animal model (100% viability). Results: Notably, the titanium adsorbed polymer matrix induced an improved healing process when compared with the individual action of each biomolecules. High-performance mass spectrometry analysis demonstrated that recovery after a traumatic event is governed by specific differentially regulated proteins, acting in a coordinated response to the external stimulus. Predicted protein interactions shown by STRING analysis were well organized in hub-based networks related with response to chemical, wound healing and response to stress pathways. Conclusions: The described functional polymer coatings of the titanium implants demonstrated the significant improvement of bone healing process after injury.
HostingRepository	PRIDE
AnnounceDate	2021-04-29
AnnouncementXML	Submission_2021-04-29_10:31:44.108.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD016135
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Felicia Antohe
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	residues isobaric at 113.084064 Da; Oxidation; L-tyrosine residue; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-04 05:17:27	ID requested
⏵ 1	2021-04-29 10:31:45	announced

Boteanu RM, Suica VI, Ivan L, Safciuc F, Uyy E, Dragan E, Croitoru SM, Grumezescu V, Chiritoiu M, Sima LE, Vlagioiu C, Socol G, Antohe F, Proteomics of regenerated tissue in response to a titanium implant with a bioactive surface in a rat tibial defect model. Sci Rep, 10(1):18493(2020) [pubmed]

submitter keyword: bone healing, growth factors, titanium implant, MS-based proteomics

Felicia Antohe
contact affiliation	Proteomics Department, Institute of Cellular Biology and Pathology "N. Simionescu", Romania
contact email	felicia.antohe@icbp.ro
lab head
Felicia Antohe
contact affiliation	Institute of Cellular Biology and Pathology "N. Simionescu"
contact email	felicia.antohe@icbp.ro
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD016135

PRIDE project URI

• PRIDE
  1. PXD016135
     1. Label: PRIDE project
     2. Name: Proteomics of regenerated tissue in response to a titanium implant with a bioactive surface in a rat tibial defect model