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PXD016133 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleElucidating the diverse roles of acetyl-CoA in the cytosol and mitochondrion of Toxoplasma gondii
DescriptionAcetyl-CoA is a key metabolite in all organisms, implicated in transcriptional regulation, post-translational modification as well as fuelling the TCA cycle and the synthesis and elongation of fatty acids. The obligate intracellular parasite Toxoplasma gondii possesses two enzymes which produce acetyl-CoA in the cytosol and nucleus: the acetyl-CoA synthase (ACS) and the ATP-citrate lyase (ACL), while the branched chain α-ketoacid dehydrogenase (BCKDH) generates acetyl-CoA in the mitochondrion. Given the diverse functions of acetyl-CoA, we know little about the specific roles of distinct sub-cellular acetyl-CoA pools and how these impact overall parasite physiology. To assess the broad functions of nucleo-cytosolic as well as mitochondrial acetyl-CoA, we analysed the acetylome and total proteome of parasites lacking ACL/ACS or BCKDH.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYohann Couté
SpeciesList scientific name: Toxoplasma gondii RH; NCBI TaxID: 383379;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-11-04 04:00:25ID requested
12020-05-07 04:31:48announced
22020-06-21 22:31:28announced2020-06-22: Updated publication reference for PubMed record(s): 32546260.
Publication List
Kloehn J, Oppenheim RD, Siddiqui G, De Bock PJ, Kumar Dogga S, Coute Y, Hakimi MA, Creek DJ, Soldati-Favre D, Multi-omics analysis delineates the distinct functions of sub-cellular acetyl-CoA pools in Toxoplasma gondii. BMC Biol, 18(1):67(2020) [pubmed]
Keyword List
submitter keyword: Toxoplasma gondii, Acetyl-CoA, metabolism, acetylome, nanoLC-MS/MS
Contact List
Virginie Brun
contact affiliationEDyP
contact emailvirginie.brun@cea.fr
lab head
Yohann Couté
contact affiliationEDyP
contact emailyohann.coute@cea.fr
dataset submitter
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