PXD016125 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The heat shock response relies on orthogonal mechanisms to maintain protein homeostasis in yeast |
Description | We globally analyzed the heat stress response (HSR) in yeast on the transcriptomic, tranlatomic and the proteomic level. The cells were stressed at 37 °C, 42 °C and 46 °C and the fractionated proteomes measured on a Fusion mass spectrometer. With this approach we were able to reveal a modular character of the HSR dependent on the severity of the stress. We showed that at temperatures up to 42 °C besides upregulation of stress protective factors refilling the pool of degraded and aggregating proteins is an important branch of the HSR. Whereas, at sublethal temperatures (46 °C) lots of aggregation and phase transition processes were observed. This is part two of the pride submission and only contains the proteomic data under inhibition of the proteasome with bortezomib. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:58:18.019.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Moritz Mühlhofer |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-11-04 03:38:05 | ID requested | |
1 | 2020-01-07 09:01:11 | announced | |
2 | 2020-01-09 10:38:37 | announced | 2020-01-09: Updated publication reference for PubMed record(s): 31875563. |
⏵ 3 | 2024-10-22 04:58:21 | announced | 2024-10-22: Updated project metadata. |
Publication List
M, ü, hlhofer M, Berchtold E, Stratil CG, Csaba G, Kunold E, Bach NC, Sieber SA, Haslbeck M, Zimmer R, Buchner J, The Heat Shock Response in Yeast Maintains Protein Homeostasis by Chaperoning and Replenishing Proteins. Cell Rep, 29(13):4593-4607.e8(2019) [pubmed] |
10.1016/j.celrep.2019.11.109; |
Keyword List
submitter keyword: chaperones,heat shock response, proteome, aggregation |
Contact List
Johannes Buchner |
contact affiliation | Technische Universität München, Dept. Chemistry, Chair of Biotechnology |
contact email | johannes.buchner@tum.de |
lab head | |
Moritz Mühlhofer |
contact affiliation | Dept. Chemie Lichtenbergstraße 4 85748 Garching |
contact email | moritz.muehlhofer@tum.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016125
- Label: PRIDE project
- Name: The heat shock response relies on orthogonal mechanisms to maintain protein homeostasis in yeast