PXD016125

PXD016125 is an original dataset announced via ProteomeXchange.

Title	The heat shock response relies on orthogonal mechanisms to maintain protein homeostasis in yeast
Description	We globally analyzed the heat stress response (HSR) in yeast on the transcriptomic, tranlatomic and the proteomic level. The cells were stressed at 37 °C, 42 °C and 46 °C and the fractionated proteomes measured on a Fusion mass spectrometer. With this approach we were able to reveal a modular character of the HSR dependent on the severity of the stress. We showed that at temperatures up to 42 °C besides upregulation of stress protective factors refilling the pool of degraded and aggregating proteins is an important branch of the HSR. Whereas, at sublethal temperatures (46 °C) lots of aggregation and phase transition processes were observed. This is part two of the pride submission and only contains the proteomic data under inhibition of the proteasome with bortezomib.
HostingRepository	PRIDE
AnnounceDate	2020-01-07
AnnouncementXML	Submission_2020-01-09_10:38:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Moritz Mühlhofer
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-04 03:38:05	ID requested
1	2020-01-07 09:01:11	announced
⏵ 2	2020-01-09 10:38:37	announced	2020-01-09: Updated publication reference for PubMed record(s): 31875563.

M, ü, hlhofer M, Berchtold E, Stratil CG, Csaba G, Kunold E, Bach NC, Sieber SA, Haslbeck M, Zimmer R, Buchner J, The Heat Shock Response in Yeast Maintains Protein Homeostasis by Chaperoning and Replenishing Proteins. Cell Rep, 29(13):4593-4607.e8(2019) [pubmed]

submitter keyword: heat shock response, aggregation, chaperones, proteome

Johannes Buchner
contact affiliation	Technische Universität München, Dept. Chemistry, Chair of Biotechnology
contact email	johannes.buchner@tum.de
lab head
Moritz Mühlhofer
contact affiliation	Dept. Chemie Lichtenbergstraße 4 85748 Garching
contact email	moritz.muehlhofer@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD016125

PRIDE project URI

• PRIDE
  1. PXD016125
     1. Label: PRIDE project
     2. Name: The heat shock response relies on orthogonal mechanisms to maintain protein homeostasis in yeast