PXD016123

PXD016123 is an original dataset announced via ProteomeXchange.

Title	Label-Free Quantitative Proteomic Profiling of LAD2 Mast Cell Releasates
Description	Tween-80 is one of the most important causes resulting in anaphylactoid reaction. However, its mechanism remains unclear. A Label-free LCMS/MS-based proteomics was used to analyze Tween-80-stimulated LAD2 mast cell releasates. Out of a total dataset of 2546 proteins, 882 proteins were found in the supernate samples of Tween-80-treated LAD2 mast cells; 313 proteins were up-expressed and 111 proteins were down-expressed. KEGG pathway analysis showed that endocytosis was the largest class of pathway. A total of 52 proteins were involved in endocytosis, and mainly related to G-protein-coupled receptor (GPCR)-endocytosis and EGFR-endocytosis. Cell adhesion molecules (CAMs) pathway was the most significant differences, with a total of 21 proteins involved. NF-κB signaling activation and calcium signaling pathway play an important role in Tween-80-induced LAD2 cells activation. Proteins, including tyrosine-protein kinase, PLCγ, PKCβ, and p50/p65, were mainly involved in NF-κB pathways. NF-κB signaling activation mainly involved in tyrosine-protein kinase, PLCγ, PKCβ, and NF-κB p50/p65. Calcium signaling pathway mainly related to PLCγ/PKC PLCγ/STIM1 mediated store-operated calcium entry (SOCE) and PLCγ mediated store-operated calcium entry (SOCE)PLCγ/PKC pathway. These results suggest that Tween-80 might be internalized via GPCR-endocytosis, which induces degranulation by SOCE PLCγ/PKC or SOCE PLCγ/PKC pathways mediated calcium influx, and promotes the generation of inflammatory mediators and CAMs via NF-κB signaling pathway finally resulting in anaphylactoid reaction.
HostingRepository	iProX
AnnounceDate	2019-11-04
AnnouncementXML	Submission_2023-08-27_21:04:12.969.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yanni Mi
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-04 02:58:54	ID requested
1	2019-11-04 03:04:55	announced
⏵ 2	2023-08-27 21:04:13	announced	2023-08-28: Update publication information.

Mi YN, Yan PP, Di J, Cao L, Xiao X, Liu DZ, Cao YX, Label-Free Quantitative Proteomic Profiling of LAD2 Mast Cell Releasates Reveals the Mechanism of Tween-80-Induced Anaphylactoid Reaction. Proteomics Clin Appl, 14(4):e1900065(2020) [pubmed]

submitter keyword: Tween-80, Anaphylactoid reaction, Proteomics, Mast cell

Yongxiao Cao
contact affiliation	Xi’an Jiaotong University
contact email	yxy@xjtu.edu.cn
lab head
Yanni Mi
contact affiliation	Xi’an Jiaotong University
contact email	yannimi@xjtu.edu.cn
dataset submitter

iProX dataset URI