PXD016123
PXD016123 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Label-Free Quantitative Proteomic Profiling of LAD2 Mast Cell Releasates |
Description | Tween-80 is one of the most important causes resulting in anaphylactoid reaction. However, its mechanism remains unclear. A Label-free LCMS/MS-based proteomics was used to analyze Tween-80-stimulated LAD2 mast cell releasates. Out of a total dataset of 2546 proteins, 882 proteins were found in the supernate samples of Tween-80-treated LAD2 mast cells; 313 proteins were up-expressed and 111 proteins were down-expressed. KEGG pathway analysis showed that endocytosis was the largest class of pathway. A total of 52 proteins were involved in endocytosis, and mainly related to G-protein-coupled receptor (GPCR)-endocytosis and EGFR-endocytosis. Cell adhesion molecules (CAMs) pathway was the most significant differences, with a total of 21 proteins involved. NF-κB signaling activation and calcium signaling pathway play an important role in Tween-80-induced LAD2 cells activation. Proteins, including tyrosine-protein kinase, PLCγ, PKCβ, and p50/p65, were mainly involved in NF-κB pathways. NF-κB signaling activation mainly involved in tyrosine-protein kinase, PLCγ, PKCβ, and NF-κB p50/p65. Calcium signaling pathway mainly related to PLCγ/PKC PLCγ/STIM1 mediated store-operated calcium entry (SOCE) and PLCγ mediated store-operated calcium entry (SOCE)PLCγ/PKC pathway. These results suggest that Tween-80 might be internalized via GPCR-endocytosis, which induces degranulation by SOCE PLCγ/PKC or SOCE PLCγ/PKC pathways mediated calcium influx, and promotes the generation of inflammatory mediators and CAMs via NF-κB signaling pathway finally resulting in anaphylactoid reaction. |
HostingRepository | iProX |
AnnounceDate | 2019-11-04 |
AnnouncementXML | Submission_2023-08-27_21:04:12.969.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yanni Mi |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-11-04 02:58:54 | ID requested | |
1 | 2019-11-04 03:04:55 | announced | |
⏵ 2 | 2023-08-27 21:04:13 | announced | 2023-08-28: Update publication information. |
Publication List
Mi YN, Yan PP, Di J, Cao L, Xiao X, Liu DZ, Cao YX, Label-Free Quantitative Proteomic Profiling of LAD2 Mast Cell Releasates Reveals the Mechanism of Tween-80-Induced Anaphylactoid Reaction. Proteomics Clin Appl, 14(4):e1900065(2020) [pubmed] |
Keyword List
submitter keyword: Tween-80, Anaphylactoid reaction, Proteomics, Mast cell |
Contact List
Yongxiao Cao | |
---|---|
contact affiliation | Xi’an Jiaotong University |
contact email | yxy@xjtu.edu.cn |
lab head | |
Yanni Mi | |
contact affiliation | Xi’an Jiaotong University |
contact email | yannimi@xjtu.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |