PXD016109

PXD016109 is an original dataset announced via ProteomeXchange.

Title	Activation of Akt, Nrf2, and NF-κB signal transduction pathways in the liver as a mechanism of survival for torpid Myotis ricketti Bats
Description	Unfolded protein accumulation in the lumen of endoplasmic reticulum (ER) induced by cold exposure is termed UPRER. Some mammals hibernate to overcome cold winter. We investigated whether hibernating bats are under UPRER and activate Akt, Nrf2, and NF-κB signaling pathways that are critical for cell survival against cold. Results of Western blotting showed that several UPRER marker proteins such as PERK and ATF4 had a higher abundance in torpid than in active bats. Cellular redistribution of GRP78 and a lower degree of binding between PERK and GRP78 were also seen in torpid bats, suggesting the occurrence of UPRER. Results also showed that torpid bats had a higher amount of p-Akt (Ser473), a higher ratio of p-Akt (Ser473)/Akt, a lower amount of Keap1/Nrf2 and NF-κB (p65)/I-κBα complexes, and a higher degree of NF-κB (p65) nuclear translocation than active bats, indicating simultaneous activation of Akt, Nrf2, and NF-κB during hibernation. Evidence of such activation was not observed in fasted, cold-treated, or normal mice. Using PPI network and IPA analyses, we examined the proteomes of liver and liver mitochondria of bats and found a global metabolic adjustment and survival adaptation in response to UPRER in hibernating bats. Our data provide the first molecular evidence of a complex cross talk involving Akt, Nrf2, and NF-κB via the PERK-EIF2-ATF4 regulatory axis under UPRER in bats to ensure their survival during hibernation.
HostingRepository	PRIDE
AnnounceDate	2020-11-17
AnnouncementXML	Submission_2020-11-16_16:16:12.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD016109
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Chen-Chung Liao
SpeciesList	scientific name: Myotis ricketti; NCBI TaxID: 203696;
ModificationList	Oxidation; Pyridylethyl
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-01 03:51:58	ID requested
⏵ 1	2020-11-16 16:16:14	announced

Huang W, Liao CC, Han Y, Lv J, Lei M, Li Y, Lv Q, Dong D, Zhang S, Pan YH, Luo J, in torpid Myotis ricketti bats for survival. Commun Biol, 3(1):658(2020) [pubmed]

submitter keyword: Hibernation, Bats, Liver, Mitochondria, Proteomics, EIF2, Akt, NF-κB, UPR, and LC-MS/MS

Chen-Chung Liao
contact affiliation	National Yang-Ming University Proteomics Research Center,Taiwan
contact email	ymliao@ym.edu.tw
lab head
Chen-Chung Liao
contact affiliation	Proteomics Research Center,National Yang-Ming University
contact email	ccliao@ym.edu.tw
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016109
     1. Label: PRIDE project
     2. Name: Activation of Akt, Nrf2, and NF-κB signal transduction pathways in the liver as a mechanism of survival for torpid Myotis ricketti Bats