PXD016102

PXD016102 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	HeLa phosphoproteome regulated by PP2A related proteins
Description	HeLa cells were treated with siRNAs of PP2A related proteins or with okadaic acid, in triplicates. Tryptic digests of extracted proteins were analyzed using a Q Exactive mass spectrometer, before and after TiO2 phosphopeptide enrichment. Data were searched with Mascot via Proteome Discoverer. For phosphorylation site localization, phosphoRS was enabled. In parallel, SpectraST searching was performed via Proteome Discoverer, against an in-house made spectral library of phosphopeptides simulated using SimPhospho software.
HostingRepository	jPOST
AnnounceDate	2020-02-19
AnnouncementXML	Submission_2022-09-18_04:05:15.484.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susumu Y. Imanishi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-31 21:02:13	ID requested
1	2020-02-19 00:58:48	announced
2	2020-02-20 02:01:42	announced	2020-02-20: Updated PubMed.
3	2021-02-04 16:58:46	announced	2021-02-04: Updated FTP location.
⏵ 4	2022-09-18 04:05:16	announced	2022-09-18: Updated FTP location.

Publication List

Kauko O, Imanishi SY, Kulesskiy E, Yetukuri L, Laajala TD, Sharma M, Pavic K, Aakula A, Rupp C, Jumppanen M, Haapaniemi P, Ruan L, Yadav B, Suni V, Varila T, Corthals GL, Reimand J, Wennerberg K, Aittokallio T, Westermarck J, Phosphoproteome and drug-response effects mediated by the three protein phosphatase 2A inhibitor proteins CIP2A, SET, and PME-1. J Biol Chem, 295(13):4194-4211(2020) [pubmed]

Kauko O, Imanishi SY, Kulesskiy E, Yetukuri L, Laajala TD, Sharma M, Pavic K, Aakula A, Rupp C, Jumppanen M, Haapaniemi P, Ruan L, Yadav B, Suni V, Varila T, Corthals GL, Reimand J, Wennerberg K, Aittokallio T, Westermarck J, Phosphoproteome and drug-response effects mediated by the three protein phosphatase 2A inhibitor proteins CIP2A, SET, and PME-1. J Biol Chem, 295(13):4194-4211(2020) [pubmed]

Keyword List

submitter keyword: PP2A, HeLa, TiO2, Q Exactive, Mascot, SpectraST, Simulated phosphopeptides

submitter keyword: PP2A, HeLa, TiO2, Q Exactive, Mascot, SpectraST, Simulated phosphopeptides

Contact List

Susumu Y. Imanishi
lab head
Susumu Y. Imanishi
contact affiliation	Meijo University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000692/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000692/