PXD016097

PXD016097 is an original dataset announced via ProteomeXchange.

Title	Discovery of potential candidate proteins reprogramming spinal cord derived astrocytes into neurons by proteomic analysis
Description	Spinal cord injury (SCI) is a common but devastating trauma of the central nerve system. In this study, we reprogrammed cultured spinal-cord reactive astrocytes into neurons by Neurod1 expression. The mechanism of programmed conversion from astrocytes to neurons has not been clarified yet. Thus, we used label-free proteomics to identify differentially expressed proteins in Neurod1 over-expressed astrocytes and control group. A total of 1952 proteins were identified, including 92 significantly changed proteins. Among these proteins, 8 proteins were identified as candidates involving in the process of the reprogramming, based on their biological function and fold change in the bioinformatic analysis. Our study revealed that that Neurod1 can directly reprogram cultured spinal-cord reactive astrocytes into neurons, and several proteins that could play a significant role during the neuronal reprogramming were discovered.
HostingRepository	PRIDE
AnnounceDate	2021-09-08
AnnouncementXML	Submission_2021-09-08_14:07:17.367.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	wenhao chen
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-31 09:58:15	ID requested
⏵ 1	2021-09-08 14:07:18	announced

Chen WH, Lin YX, Lin L, Zhang BQ, Xu SX, Wang W, Identification of potential candidate proteins for reprogramming spinal cord-derived astrocytes into neurons: a proteomic analysis. Neural Regen Res, 16(11):2257-2263(2021) [pubmed]

submitter keyword: spinal cord injury, reprogramming, astrocytes, neurons, label-free proteomic analysis

wenhao chen
contact affiliation	Fujian medical university union hospital
contact email	15606075620@163.com
lab head
wenhao chen
contact affiliation	Fujian medical university union hospital
contact email	15606075620@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016097
     1. Label: PRIDE project
     2. Name: Discovery of potential candidate proteins reprogramming spinal cord derived astrocytes into neurons by proteomic analysis