PXD016089 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of novel blood biomarkers of treatment response in cystic fibrosis pulmonary exacerbations by label-free quantitative proteomics |
Description | Pulmonary exacerbations (PEx) are clinically impactful events for individuals with CF. Unfortunately, many CF individuals with PEx fail to regain their baseline lung function despite treatment. The objective of this study was to use unbiased proteomic technology (LC-MS/MS) to identify novel blood protein biomarkers that change following intravenous (IV) antibiotic treatment and to explore if changes correlate with clinical response by the end of treatment. Blood samples from 25 PEx events derived from 22 unique CF adults were collected within 24 hours of hospital admission, day 5, day 10, and IV antibiotic completion. Three-hundred and forty-six blood proteins were evaluated with label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS) quantitative proteomics and immunoassays. Forty-seven plasma proteins changed significantly following 5 days of IV antibiotic treatment (q-value ≤ 0.10). Early change in IGF2R from hospital admission to day 5 correlated with overall change in symptom score (CFRSD-CRISS) by the end of treatment (r = -0.48, p-value = 0.04). Several plasma proteins identified and quantified by label-free LC-MS/MS changed early following treatment with IV antibiotics and many of these proteins are involved in complement activation and inflammatory/immune-related pathways. Early change in IGF2R correlated with symptom response following IV antibiotic treatment and requires further validation as a predictive biomarker of symptom response. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:06:11.282.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jenny Moon |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Bruker Daltonics instrument model |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-10-31 02:17:11 | ID requested | |
1 | 2020-05-26 19:10:50 | announced | |
⏵ 2 | 2024-10-22 05:06:15 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Human, cystic fibrosis, plasma, blood, pulmonary exacerbations, LC-MS/MS |
Contact List
Bradley S. Quon |
contact affiliation | Centre for Heart Lung Innovation, University of British Columbia, Vancouver, BC, Canada Division of Respiratory Medicine, Department of Medicine, University of British Columbia, Vancouver, BC, Canada |
contact email | bradley.quon@hli.ubc.ca |
lab head | |
Jenny Moon |
contact affiliation | University of British Columbia |
contact email | kyungmee@mail.ubc.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016089
- Label: PRIDE project
- Name: Identification of novel blood biomarkers of treatment response in cystic fibrosis pulmonary exacerbations by label-free quantitative proteomics