PXD016072

PXD016072 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomic Analysis of buffalo ovaries
Description	The ovary is the most important sexual organ of female mammals, and its corpus luteum plays a significant role in mammalian reproduction during the estrus cycle. Here we conducted a quantitative proteomic analysis using tandem mass tag (TMT)-labeling coupled with Nano-LC-MS/MS to explore the mechanisms of the estrus cycle in protein level. A total of 2,103 proteins were quantified in three groups, corpus hemorrhagicum (CH), corpus luteum (CL), and corpus fibrosum (CF). Thirty-two proteins were identified as differentially expressed in the CH group, and 116 proteins were identified as differentially expressed in the CF group, with the CL group serving as the control. Notably, we found that many enzymes, including kinase and phosphatase, are upregulated in the CL stage of the ovary, and three upregulated proteins in the CL stage (PLK1, PGP, and HGS) were confirmed using western blotting, quantitative RT-PCR, and immunohistochemistry analysis. The results of these validations were consistent with the TMT-label quantitative analysis, which indicated that they may play a crucial role in the normal reproductive cycle of the CL. Our study not only provides a deeper understanding of the formation and regression of the CL, but also suggests some potential proteins related to the estrus cycle.
HostingRepository	iProX
AnnounceDate	2019-10-30
AnnouncementXML	Submission_2019-12-09_20:49:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fumei Chen
SpeciesList	scientific name: Bubalus bubalis; NCBI TaxID: 89462;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-29 20:31:10	ID requested
1	2019-10-29 20:35:34	announced
⏵ 2	2019-12-09 20:49:58	announced	2019-12-10: Update publication information.

Fu Q, Huang Y, Wang Z, Chen F, Huang D, Lu Y, Liang X, Zhang M, Proteome Profile and Quantitative Proteomic Analysis of Buffalo (Bubalusbubalis) Follicular Fluid during Follicle Development. Int J Mol Sci, 17(5):(2016) [pubmed]

submitter keyword: corpus luteum ,ovary, LC-MS/MS, TMT, estrus cycle

Ming Zhang
contact affiliation	State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresource, Animal Reproduction Institute, Guangxi University
contact email	mingzhang@gxu.edu.cn
lab head
Fumei Chen
contact affiliation	State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresource
contact email	chen.3009@163.com
dataset submitter

iProX dataset URI